RGD Reference Report - Effects of receptor-mediated endocytosis and tubular protein composition on volume retention in experimental glomerulonephritis. - Rat Genome Database

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Effects of receptor-mediated endocytosis and tubular protein composition on volume retention in experimental glomerulonephritis.

Authors: Kastner, C  Pohl, M  Sendeski, M  Stange, G  Wagner, CA  Jensen, B  Patzak, A  Bachmann, S  Theilig, F 
Citation: Kastner C, etal., Am J Physiol Renal Physiol. 2009 Apr;296(4):F902-11. doi: 10.1152/ajprenal.90451.2008. Epub 2009 Feb 4.
RGD ID: 7242947
Pubmed: PMID:19193726   (View Abstract at PubMed)
DOI: DOI:10.1152/ajprenal.90451.2008   (Journal Full-text)

Human glomerulonephritis (GN) is characterized by sustained proteinuria, sodium retention, hypertension, and edema formation. Increasing quantities of filtered protein enter the renal tubule, where they may alter epithelial transport functions. Exaggerated endocytosis and consequent protein overload may affect proximal tubules, but intrinsic malfunction of distal epithelia has also been reported. A straightforward assignment to a particular tubule segment causing salt retention in GN is still controversial. We hypothesized that 1) trafficking and surface expression of major transporters and channels involved in volume regulation were altered in GN, and 2) proximal tubular endocytosis may influence locally as well as downstream expressed tubular transporters and channels. Effects of anti-glomerular basement membrane GN were studied in controls and megalin-deficient mice with blunted proximal endocytosis. Mice displayed salt retention and elevated systolic blood pressure when proteinuria had reached 10-15 mg/24 h. Surface expression of proximal Na(+)-coupled transporters and water channels was in part [Na(+)-P(i) cotransporter IIa (NaPi-IIa) and aquaporin-1 (AQP1)] increased by megalin deficiency alone, but unchanged (Na(+)/H(+) exchanger 3) or reduced (NaPi-IIa and AQP1) in GN irrespective of the endocytosis defect. In distal epithelia, significant increases in proteolytic cleavage products of alpha-epithelial Na(+) channel (ENaC) and gamma-ENaC were observed, suggesting enhanced tubular sodium reabsorption. The effects of glomerular proteinuria dominated over those of blunted proximal endocytosis in contributing to ENaC cleavage. Our data indicate that ENaC-mediated sodium entry may be the rate-limiting step in proteinuric sodium retention. Enhanced proteolytic cleavage of ENaC points to a novel mechanism of channel activation which may involve the action of filtered plasma proteases.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
anti-basement membrane glomerulonephritis  ISOSlc34a1 (Mus musculus)7242947; 7242947protein:decreased expression:kidney and brush border membrane (mouse)RGD 
anti-basement membrane glomerulonephritis  IEP 7242947protein:decreased expression:kidney and brush border membrane (mouse)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc34a1  (solute carrier family 34 member 1)

Genes (Mus musculus)
Slc34a1  (solute carrier family 34 (sodium phosphate), member 1)

Genes (Homo sapiens)
SLC34A1  (solute carrier family 34 member 1)


Additional Information