RGD Reference Report - Laser microdissection and mass spectrometry-based proteomics aids the diagnosis and typing of renal amyloidosis. - Rat Genome Database

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Laser microdissection and mass spectrometry-based proteomics aids the diagnosis and typing of renal amyloidosis.

Authors: Sethi, S  Vrana, JA  Theis, JD  Leung, N  Sethi, A  Nasr, SH  Fervenza, FC  Cornell, LD  Fidler, ME  Dogan, A 
Citation: Sethi S, etal., Kidney Int. 2012 Jul;82(2):226-34. doi: 10.1038/ki.2012.108. Epub 2012 Apr 11.
RGD ID: 7241855
Pubmed: PMID:22495291   (View Abstract at PubMed)
PMCID: PMC3388518   (View Article at PubMed Central)
DOI: DOI:10.1038/ki.2012.108   (Journal Full-text)

Accurate diagnosis and typing of renal amyloidosis is critical for prognosis, genetic counseling, and treatment. Laser microdissection and mass spectrometry are emerging techniques for the analysis and diagnosis of many renal diseases. Here we present the results of laser microdissection and mass spectrometry performed on 127 cases of renal amyloidosis during 2008-2010. We found the following proteins in the amyloid deposits: immunoglobulin light and heavy chains, secondary reactive serum amyloid A protein, leukocyte cell-derived chemotaxin-2, fibrinogen-alpha chain, transthyretin, apolipoprotein A-I and A-IV, gelsolin, and beta-2 microglobulin. Thus, laser microdissection of affected areas within the kidney followed by mass spectrometry provides a direct test of the composition of the deposit and forms a useful ancillary technique for the accurate diagnosis and typing of renal amyloidosis in a single procedure.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Familial Amyloidosis  IEP 7241855 RGD 
Familial Amyloidosis  ISOAPOA1 (Homo sapiens)7241855; 7241855 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Apoa1  (apolipoprotein A1)

Genes (Mus musculus)
Apoa1  (apolipoprotein A-I)

Genes (Homo sapiens)
APOA1  (apolipoprotein A1)


Additional Information