RGD Reference Report - Inhibition of aldehyde dehydrogenase 2 activity enhances antimycin-induced rat cardiomyocytes apoptosis through activation of MAPK signaling pathway. - Rat Genome Database

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Inhibition of aldehyde dehydrogenase 2 activity enhances antimycin-induced rat cardiomyocytes apoptosis through activation of MAPK signaling pathway.

Authors: Zhang, P  Xu, D  Wang, S  Fu, H  Wang, K  Zou, Y  Sun, A  Ge, J 
Citation: Zhang P, etal., Biomed Pharmacother. 2011 Dec;65(8):590-3. doi: 10.1016/j.biopha.2009.12.001. Epub 2009 Dec 30.
RGD ID: 7241589
Pubmed: PMID:21123025   (View Abstract at PubMed)
DOI: DOI:10.1016/j.biopha.2009.12.001   (Journal Full-text)

Aldehyde dehydrogenase 2 (ALDH2), a mitochondrial-specific enzyme, has been proved to be involved in oxidative stress-induced cell apoptosis, while little is known in cardiomyocytes. This study was aimed at investigating the role of ALDH2 in antimycin A-induced cardiomyocytes apoptosis by suppressing ALDH2 activity with a specific ALDH2 inhibitor Daidzin. Antimycin A (40mug/ml) was used to induce neonatal cardiomyocytes apoptosis. Daidzin (60muM) effectively inhibited ALDH2 activity by 50% without own effect on cell apoptosis, and significantly enhanced antimycin A-induced cardiomyocytes apoptosis from 33.5+/-4.4 to 56.5+/-6.4% (Hochest method, p<0.05), and from 57.9+/-1.9 to 74.0+/-11.9% (FACS, p<0.05). Phosphorylation of activated MAPK signaling pathway, including extracellular signal-regulated kinase (ERK1/2), c-Jun NH2-terminal kinase (JNK) and p38 was also increased in antimycin A and daidzin treated cardiomyocytes compared to the cells treated with antimycin A alone. These findings indicated that modifying mitochondrial ALDH2 activity/expression might be a potential therapeutic option on reducing oxidative insults induced cardiomyocytes apoptosis.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
negative regulation of apoptotic process  IMP 7241589 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Aldh2  (aldehyde dehydrogenase 2 family member)


Additional Information