RGD Reference Report - Serial non-invasive assessment of antibody induced nephritis in mice using positron emission tomography. - Rat Genome Database

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Serial non-invasive assessment of antibody induced nephritis in mice using positron emission tomography.

Authors: Hao, G  Du, Y  Zhou, XJ  Guo, J  Sun, X  Mohan, C  Oz, OK 
Citation: Hao G, etal., PLoS One. 2013;8(2):e57418. doi: 10.1371/journal.pone.0057418. Epub 2013 Feb 27.
RGD ID: 7241232
Pubmed: PMID:23460853   (View Abstract at PubMed)
PMCID: PMC3584026   (View Article at PubMed Central)
DOI: DOI:10.1371/journal.pone.0057418   (Journal Full-text)

Mouse models of experimental anti-glomerular basement membrane (anti-GBM) nephritis provide an analytical tool for studying spontaneous lupus nephritis. The potential of Positron Emission Tomography (PET) was evaluated using 2-deoxy-2-[F]fluoro-d-glucose (FDG) as a probe to monitor the progression of anti-GBM induced nephritis in a mouse model. The imaging results were compared to conventional measures of renal function and pathological changes. Serum and urinary vascular cell adhesion molecule-1 (VCAM-1) levels were used as measures of endothelial cell activation and inflammation. Following a challenge with anti-glomerular antibodies, mice exhibited peak changes in serum creatinine, proteinuria, and glomerulonephritis score at 14 days post-challenge (p.c.). In contrast, VCAM levels peaked at day 7 p.c. On dynamic PET images (0-60 min) of day 7, kidneys of the anti-GBM nephritis mice demonstrated a unique pattern of FDG uptake. Compared to the time activity curve (TAC) prior to challenge, a rightward shift was observed after the challenge. By day 10 p.c., kidney FDG uptake was lower than baseline and remained so until the study ended at 21 days p.c. During this time frame measures of renal dysfunction remained high but VCAM-1 levels declined. These changes were accompanied by an increase in kidney volume as measured by Computed Tomography (CT) and intra-abdominal fluid collection. Our results suggest that FDG-PET-CT can be used as a non-invasive imaging tool to longitudinally monitor the progression of renal disease activity in antibody mediated nephritis and the magnitude of renal FDG retention correlates better with early markers of renal inflammation than renal dysfunction.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
anti-basement membrane glomerulonephritis  ISOVcam1 (Mus musculus)7241232; 7241232protein:increased expression:urine and serum:RGD 
anti-basement membrane glomerulonephritis  IEP 7241232protein:increased expression:urine and serum:RGD 

Objects Annotated

Genes (Rattus norvegicus)
Vcam1  (vascular cell adhesion molecule 1)

Genes (Mus musculus)
Vcam1  (vascular cell adhesion molecule 1)

Genes (Homo sapiens)
VCAM1  (vascular cell adhesion molecule 1)


Additional Information