RGD Reference Report - MMP profile in paired serum and synovial fluid samples of patients with rheumatoid arthritis. - Rat Genome Database

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MMP profile in paired serum and synovial fluid samples of patients with rheumatoid arthritis.

Authors: Tchetverikov, I  Ronday, HK  Van El, B  Kiers, GH  Verzijl, N  TeKoppele, JM  Huizinga, TW  DeGroot, J  Hanemaaijer, R 
Citation: Tchetverikov I, etal., Ann Rheum Dis. 2004 Jul;63(7):881-3.
RGD ID: 7207131
Pubmed: PMID:15194590   (View Abstract at PubMed)
PMCID: PMC1755080   (View Article at PubMed Central)
DOI: DOI:10.1136/ard.2003.013243   (Journal Full-text)

OBJECTIVE: To analyse matrix metalloproteinases (MMPs) and tissue inhibitor-1 of MMPs (TIMP-1) levels in the systemic circulation and synovial fluid (SF) of patients with RA and to compare these levels with inflammatory and collagen degradation markers. METHODS: ProMMP-1, -2, -3, -8, -9, TIMP-1, levels of MMP/alpha(2)-macroglobulin complexes, and collagen degradation products were measured by sandwich ELISA, activity assays, and HPLC in paired SF and serum samples from 15 patients with RA and 13 with OA. RESULTS: MMPs were higher in SF of patients with RA than in OA or controls. MMP levels in SF of patients with OA were higher than in controls. In serum, levels of proMMP-3, -8 and -9 were higher in patients with RA than in OA or controls, whereas only proMMP-8 and -9 were higher in serum of patients with OA than in controls. A strong correlation was seen between serum and SF levels of MMP-8 and -9 in RA. Increased levels of MMP/alpha(2)-macroglobulin complexes indicated an MMP/TIMP imbalance in serum and SF in RA. SF hydroxyproline correlated significantly with SF levels of proMMP-9 in RA. CONCLUSIONS: Systemic MMP-8 and -9 levels represent the situation in the inflamed joint; MMP-9 is likely to be involved in degradation of joint collagen. The hypothesis of MMP/TIMP imbalance in RA is strengthened.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
osteoarthritis  IEP 7207131; 7207131protein:increased expression:synovial fluid (human)RGD 
osteoarthritis  IEP 7207131; 7207131protein:increased expression:synovial fluid and serum (human)RGD 
osteoarthritis  ISOMMP2 (Homo sapiens)7207131; 7207131protein:increased expression:synovial fluid (human)RGD 
osteoarthritis  ISOMMP3 (Homo sapiens)7207131; 7207131protein:increased expression:synovial fluid (human)RGD 
osteoarthritis  ISOMMP8 (Homo sapiens)7207131; 7207131protein:increased expression:synovial fluid and serum (human)RGD 
osteoarthritis  ISOMMP9 (Homo sapiens)7207131; 7207131protein:increased expression:synovial fluid and serum (human)RGD 
rheumatoid arthritis  IEP 7207131protein:increased expression:synovial fluid and serum (human)RGD 
rheumatoid arthritis  ISOMMP8 (Homo sapiens)7207131; 7207131protein:increased expression:synovial fluid and serum (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mmp2  (matrix metallopeptidase 2)
Mmp3  (matrix metallopeptidase 3)
Mmp8  (matrix metallopeptidase 8)
Mmp9  (matrix metallopeptidase 9)

Genes (Mus musculus)
Mmp2  (matrix metallopeptidase 2)
Mmp3  (matrix metallopeptidase 3)
Mmp8  (matrix metallopeptidase 8)
Mmp9  (matrix metallopeptidase 9)

Genes (Homo sapiens)
MMP2  (matrix metallopeptidase 2)
MMP3  (matrix metallopeptidase 3)
MMP8  (matrix metallopeptidase 8)
MMP9  (matrix metallopeptidase 9)


Additional Information