RGD Reference Report - Profiling and identification of cerebrospinal fluid proteins in a rat EAE model of multiple sclerosis. - Rat Genome Database

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Profiling and identification of cerebrospinal fluid proteins in a rat EAE model of multiple sclerosis.

Authors: Rosenling, T  Stoop, MP  Attali, A  Van Aken, H  Suidgeest, E  Christin, C  Stingl, C  Suits, F  Horvatovich, P  Hintzen, RQ  Tuinstra, T  Bischoff, R  Luider, TM 
Citation: Rosenling T, etal., J Proteome Res. 2012 Apr 6;11(4):2048-60. Epub 2012 Feb 23.
RGD ID: 7175513
Pubmed: PMID:22320401   (View Abstract at PubMed)
DOI: DOI:10.1021/pr201244t   (Journal Full-text)

The experimental autoimmune encephalomyelitis (EAE) model resembles certain aspects of multiple sclerosis (MScl), with common features such as motor dysfunction, axonal degradation, and infiltration of T-cells. We studied the cerebrospinal fluid (CSF) proteome in the EAE rat model to identify proteomic changes relevant for MScl disease pathology. EAE was induced in male Lewis rats by injection of myelin basic protein (MBP) together with complete Freund's adjuvant (CFA). An inflammatory control group was injected with CFA alone, and a nontreated group served as healthy control. CSF was collected at day 10 and 14 after immunization and analyzed by bottom-up proteomics on Orbitrap LC-MS and QTOF LC-MS platforms in two independent laboratories. By combining results, 44 proteins were discovered to be significantly increased in EAE animals compared to both control groups, 25 of which have not been mentioned in relation to the EAE model before. Lysozyme C1, fetuin B, T-kininogen, serum paraoxonase/arylesterase 1, glutathione peroxidase 3, complement C3, and afamin are among the proteins significantly elevated in this rat EAE model. Two proteins, afamin and complement C3, were validated in an independent sample set using quantitative selected reaction monitoring mass spectrometry. The molecular weights of the identified differentially abundant proteins indicated an increased transport across the blood-brain barrier (BBB) at the peak of the disease, caused by an increase in BBB permeability.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Experimental Autoimmune Encephalomyelitis  IEP 7175513protein:increased expression:cerebrospinal fluidRGD 
Experimental Autoimmune Encephalomyelitis  ISOC3 (Rattus norvegicus)7175513; 7175513protein:increased expression:cerebrospinal fluidRGD 
Experimental Autoimmune Encephalomyelitis  ISOGpx3 (Rattus norvegicus)7175513; 7175513 RGD 
Experimental Autoimmune Encephalomyelitis  IEP 7175513 RGD 

Objects Annotated

Genes (Rattus norvegicus)
C3  (complement C3)
Gpx3  (glutathione peroxidase 3)

Genes (Mus musculus)
C3  (complement component 3)
Gpx3  (glutathione peroxidase 3)

Genes (Homo sapiens)
C3  (complement C3)
GPX3  (glutathione peroxidase 3)


Additional Information