RGD Reference Report - Organelles containing inositol trisphosphate receptor type 2 in adrenal medullary cells. - Rat Genome Database

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Organelles containing inositol trisphosphate receptor type 2 in adrenal medullary cells.

Authors: Endo, Y  Harada, K  Fujishiro, N  Funahashi, H  Shioda, S  Prestwich, GD  Mikoshiba, K  Inoue, M 
Citation: Endo Y, etal., J Physiol Sci. 2006 Dec;56(6):415-23. Epub 2006 Nov 3.
RGD ID: 7175267
Pubmed: PMID:17081354   (View Abstract at PubMed)
DOI: DOI:10.2170/physiolsci.RP006406   (Journal Full-text)

To identify which organelles contained inositol trisphosphate (InsP(3)) receptor type 2 (InsP(3)R2) in adrenal medullary (AM) cells, immunocytochemical and biochemical studies were performed on AM cells of several species. InsP(3)R2-like immunoreactive materials produced by two different anti-InsP(3)R2 antibodies (Abs) (Chemicon and Sigma) were distributed in rat AM cells in agreement with BODIPY-FL-InsP(3) binding sites. For two other Abs (KM1083 and Santa Cruz), some of the anti-InsP(3)R2 immunoreactive materials were stained with an anti-dopamine-beta-hydroxylase Ab, but not by BODIPY-FL-InsP(3). BODIPY-FL-thapsigargin binding sites were consistent with a distribution of the endoplasmic reticulum (ER) identified by an anti-calnexin Ab, and a prior application of thapsigargin significantly eliminated BODIPY-FL-thapsigargin bindings, suggesting that BODIPY-FL-thapsigargin bindings were mediated by thapsigargin, but not the fluorescence molecule. The anti-InsP(3)R2 Ab that produced stainings consistent with BODIPY-FL-InsP(3) bindings recognized a protein with about 250 kDa. A fractional analysis of bovine adrenal medullae revealed that the 250 kDa InsP(3)R2 was detected in a crude membrane fraction, but not in a secretory granule fraction. The results suggest that the InsP(3)R2 was present in the ER, but not in secretory granules in AM cells.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
inositol 1,4,5 trisphosphate binding  IDA 7175267 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Itpr2  (inositol 1,4,5-trisphosphate receptor, type 2)


Additional Information