RGD Reference Report - Growth factor regulation of uncoupling protein-1 mRNA expression in brown adipocytes. - Rat Genome Database

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Growth factor regulation of uncoupling protein-1 mRNA expression in brown adipocytes.

Authors: Garcia, B  Obregon, MJ 
Citation: Garcia B and Obregon MJ, Am J Physiol Cell Physiol 2002 Jan;282(1):C105-12.
RGD ID: 70670
Pubmed: PMID:11742803   (View Abstract at PubMed)
DOI: DOI:10.1152/ajpcell.01396.2000   (Journal Full-text)

To study the effect of the mitogens epidermal growth factor (EGF), acidic and basic fibroblast growth factors (aFGF and bFGF), and vasopressin on brown adipocyte differentiation, we analyzed the expression of uncoupling protein-1 (UCP-1) mRNA. Quiescent brown preadipocytes express high levels of UCP-1 mRNA in response to triiodothyronine (T3) and norepinephrine (NE). The addition of serum or the mitogenic condition aFGF + vasopressin + NE or EGF + vasopressin + NE decreases UCP-1 mRNA. A second addition of mitogens further decreases UCP-1 mRNA. Treatment with aFGF or bFGF alone increases UCP-1 mRNA, whereas the addition of EGF or vasopressin dramatically reduces UCP-1 mRNA levels. The continuous presence of T3 increases UCP-1 mRNA levels in cells treated with EGF, aFGF, or bFGF. The effect of T3 on the stimulation of DNA synthesis also was tested. T3 inhibits the mitogenic activity of aFGF and bFGF. In conclusion, mitogens like aFGF or bFGF allow brown adipocyte differentiation, whereas EGF and vasopressin inhibit the differentiation process. T3 behaves as an important hormone that regulates both brown adipocyte proliferation and differentiation.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
generation of precursor metabolites and energy  TAS 70670 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
mitochondrial inner membrane  TAS 70670 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ucp1  (uncoupling protein 1)


Additional Information