RGD Reference Report - Molecular and functional characterization of acid-sensing ion channel (ASIC) 1b. - Rat Genome Database

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Molecular and functional characterization of acid-sensing ion channel (ASIC) 1b.

Authors: Bassler, EL  Ngo-Anh, TJ  Geisler, HS  Ruppersberg, JP  Grunder, S 
Citation: Bassler EL, etal., J Biol Chem 2001 Sep 7;276(36):33782-7.
RGD ID: 70652
Pubmed: PMID:11448963   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M104030200   (Journal Full-text)

Acid-sensing ion channels (ASICs) are activated by extracellular protons and are involved in neurotransmission in the central nervous system, in pain perception, as well as in mechanotransduction. Six different ASIC subunits have been cloned to date, which are encoded by four genes (ASIC1-ASIC4). Proton-gated currents have been described in isolated neurons from sensory ganglia as well as from central nervous system. However, it is largely unclear which of the cloned ASIC subunits underlie these native proton-gated currents. Recently, a splice variant, ASIC-beta, has been described for ASIC1a. In this variant about one-third of the protein is exchanged at the N terminus. Here we show that ASIC-beta has a longer N terminus than previously reported, extending the sequence divergence between ASIC1a and this new variant (ASIC1b). We investigated in detail kinetic and selectivity properties of ASIC1b in comparison to ASIC1a. Kinetics is similar for ASIC1b and ASIC1a. Ca(2+) permeability of ASIC1a is low, whereas ASIC1b is impermeable to Ca(2+). Currents through ASIC1a resemble currents, which have been described in sensory and central neurons, whereas the significance of ASIC1b remains to be established. Moreover, we show that a pre-transmembrane 1 domain controls the permeability to divalent cations in ASIC1, contributing to our understanding of the pore structure of these channels.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
inorganic cation transmembrane transport acts_upstream_of_or_withinIDA 70652PMID:11448963MGI 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
membrane located_inIDA 70652PMID:11448963MGI 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
inorganic cation transmembrane transporter activity enablesIDA 70652PMID:11448963MGI 
pH-gated monoatomic ion channel activity enablesIDA 70652PMID:11448963UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Asic1  (acid sensing ion channel subunit 1)

Objects referenced in this article
Gene Asic1 acid-sensing ion channel 1 Mus musculus

Additional Information