RGD Reference Report - In vitro induction of bilirubin conjugation in primary rat hepatocyte culture. - Rat Genome Database

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In vitro induction of bilirubin conjugation in primary rat hepatocyte culture.

Authors: Jemnitz, K  Lengyel, G  Vereczkey, L 
Citation: Jemnitz K, etal., Biochem Biophys Res Commun 2002 Feb 15;291(1):29-33.
RGD ID: 70480
Pubmed: PMID:11829457   (View Abstract at PubMed)
DOI: DOI:10.1006/bbrc.2002.6400   (Journal Full-text)

UDP-glucuronosyltransferase (UGT1A1) is a critical enzyme in the elimination of bilirubin. The aim of our study was to investigate bilirubin conjugation in primary rat hepatocyte culture and the in vitro inducibility of this isoenzyme by inducing compounds of different classes: dexamethasone, clofibrate, rifampicin, and methylcholanthrene. Hepatocytes exhibited a marked decline in UGT1A1 activity in the first 4 h of culturing (10% of initial activity) and the recovery took 72 h. Immunoblot analysis proved that the loss of enzyme activity was associated with the decrease of protein concentration. Marked induction was detected in the cases of dexamethasone, clofibrate, and rifampicin treatments for 96 h both in enzyme activity (178, 176, and 168%) and in UGT1A1 protein level (362, 328, and 250%). The effects of dexamethasone and clofibrate were additive (210%). Methylcholanthrene had no influence on bilirubin conjugation in our system.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
bilirubin conjugation  TAS 70480 RGD 
response to glucocorticoid  IEP 70480 RGD 
response to xenobiotic stimulus  IEP 70480 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
glucuronosyltransferase activity  IDA 70480 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ugt1a1  (UDP glucuronosyltransferase family 1 member A1)


Additional Information