RGD Reference Report - Isolation and characterization of mouse homolog of the neutrophil activating peptide-2. - Rat Genome Database

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Isolation and characterization of mouse homolog of the neutrophil activating peptide-2.

Authors: Oda, M  Haruta, H  Nagao, M  Nagata, Y 
Citation: Oda M, etal., Biochem Biophys Res Commun 2002 Jan 18;290(2):865-8.
RGD ID: 70252
Pubmed: PMID:11785982   (View Abstract at PubMed)
DOI: DOI:10.1006/bbrc.2001.6279   (Journal Full-text)

In the presence of thrombopoietin (TPO), megakaryocytes mature by polyploidization and cytoplasmic maturation, and the matured megakaryocytes induce drastic morphological change and proplatelet formation and release a number of platelets. However, the regulatory mechanism of this unique differentiation process is still obscure. We therefore attempted to identify the factors, expression of which is induced by TPO stimulation in mouse bone marrow megakaryocytes. We isolated the mouse homolog of the neutrophil activating peptide-2 (NAP-2). Mouse NAP-2 cDNA encodes a predicted sequence of 113 amino acids and contains the Cys motif (CXC) found in other members of the alpha-chemokine family. At the amino acid level, the predicted mouse NAP-2 has 50.4%, 51.8%, and 72.6% identity with the predicted human, pig, and rat NAP-2, respectively. Northern blot analysis demonstrates that mouse NAP-2 is expressed only in spleen. Furthermore, the RT-PCR technique shows that the mouse NAP-2 gene is clearly upregulated by TPO stimulation in mouse megakaryocytes.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
platelet formation  TAS 70252 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ppbp  (pro-platelet basic protein)

Objects referenced in this article
Gene Ppbp pro-platelet basic protein Mus musculus

Additional Information