RGD Reference Report - Cloning and expression of a rat brain L-glutamate transporter. - Rat Genome Database

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Cloning and expression of a rat brain L-glutamate transporter.

Authors: Pines, G  Danbolt, NC  Bjoras, M  Zhang, Y  Bendahan, A  Eide, L  Koepsell, H  Storm-Mathisen, J  Seeberg, E  Kanner, BI 
Citation: Pines G, etal., Nature 1992 Dec 3;360(6403):464-7.
RGD ID: 70006
Pubmed: PMID:1448170   (View Abstract at PubMed)
DOI: DOI:10.1038/360464a0   (Journal Full-text)

Synaptic transmission of most vertebrate synapses is thought to be terminated by rapid transport of the neurotransmitter into presynaptic nerve terminals or neuroglia. L-Glutamate is the major excitatory transmitter in brain and its transport represents the mechanism by which it is removed from the synaptic cleft and kept below toxic levels. Here we use an antibody against a glial L-glutamate transporter from rat brain to isolate a complementary DNA clone encoding this transporter. Expression of this cDNA in transfected HeLa cells indicates that L-glutamate accumulation requires external sodium and internal potassium and transport shows the expected stereospecificity. The cDNA sequence predicts a protein of 573 amino acids with 8-9 putative transmembrane alpha-helices. Database searches indicate that this protein is not homologous to any identified protein of mammalian origin, including the recently described superfamily of neurotransmitter transporters. This protein therefore seems to be a member of a new family of transport molecules.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
plasma membrane located_inIMP 70006PMID:1448170UniProt 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
glutamate:sodium symporter activity enablesIDA 70006PMID:1448170UniProt 
high-affinity L-glutamate transmembrane transporter activity enablesIDA 70006PMID:1448170UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Slc1a2  (solute carrier family 1 member 2)

Objects referenced in this article
Gene SLC1A2 solute carrier family 1 member 2 Homo sapiens

Additional Information