RGD Reference Report - Cloning and characterization of the cDNAs for human and rat corticotropin releasing factor-binding proteins. - Rat Genome Database

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Cloning and characterization of the cDNAs for human and rat corticotropin releasing factor-binding proteins.

Authors: Potter, E  Behan, DP  Fischer, WH  Linton, EA  Lowry, PJ  Vale, WW 
Citation: Potter E, etal., Nature 1991 Jan 31;349(6308):423-6.
RGD ID: 69816
Pubmed: PMID:1846945   (View Abstract at PubMed)
DOI: DOI:10.1038/349423a0   (Journal Full-text)

Corticotropin-releasing factor (CRF), is a potent stimulator of synthesis and secretion of preopiomelanocortin-derived peptides. Although CRF concentrations in the human peripheral circulation are normally low, they increase throughout pregnancy and fall rapidly after parturition. Maternal plasma CRF probably originates from the placenta, which responds to the bioactive peptide and produces the peptide and its messenger RNA. Even though CRF concentrations in late gestational maternal plasma are similar to those in rat hypothalamic portal blood and to those that can stimulate release of adrenocorticotropic hormone (ACTH) in vitro, maternal plasma ACTH concentrations increase only slightly with advancing gestation and remain within the normal range. Several groups have now reported the existence of a CRF-binding protein in human plasma which inactivates CRF and which has been proposed to prevent inappropriate pituitary-adrenal stimulation in pregnancy. The binding protein was recently purified from human plasma. We have now isolated and partially sequenced the binding protein, allowing us to clone and characterize its complementary DNA from human liver and rat brain. Expression of the cDNAs for human and rat binding protein in COS7 cells showed that these proteins bind CRF with the same affinity as the native human protein. Both rat and human recombinant binding proteins inhibit CRF binding to a CRF antibody and inhibit CRF-induced ACTH release by pituitary cells in vitro.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
negative regulation of corticotropin secretion involved_inIDA 69816PMID:1846945UniProt 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
extracellular space  IDA 69816 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
corticotropin-releasing hormone binding  IDA 69816 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Crhbp  (corticotropin releasing hormone binding protein)

Objects referenced in this article
Gene Chad chondroadherin Rattus norvegicus

Additional Information