RGD Reference Report - Endogenous adipocyte apolipoprotein E is colocalized with caveolin at the adipocyte plasma membrane. - Rat Genome Database

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Endogenous adipocyte apolipoprotein E is colocalized with caveolin at the adipocyte plasma membrane.

Authors: Yue, L  Mazzone, T 
Citation: Yue L and Mazzone T, J Lipid Res. 2011 Mar;52(3):489-98. Epub 2010 Dec 16.
RGD ID: 6904144
Pubmed: PMID:21169230   (View Abstract at PubMed)
PMCID: PMC3035685   (View Article at PubMed Central)
DOI: DOI:10.1194/jlr.M011809   (Journal Full-text)

Apolipoprotein (apo)E is well established as a secreted protein that plays an important role in systemic lipoprotein metabolism and vascular wall homeostasis. Recently, endogenous expression of apoE in adipocytes has been shown to play an important role in adipocyte lipoprotein metabolism and gene expression consistent with a nonsecreted cellular itinerary for apoE. We designed studies to evaluate if adipocyte apoE was retained as a constituent protein in adipocytes and to identify a cellular retention compartment. Using confocal microscopy, coimmunoprecipitation, and sucrose density cellular fractionation, we establish that endogenous apoE shares a cellular itinerary with the constituent protein caveolin-1. Altering adipocyte caveolar number by modulating cellular cholesterol flux or altering caveolin expression regulates the distribution of cellular apoE between cytoplasmic and plasma membrane compartments. A mechanism for colocalization of apoE with caveolin was established by demonstrating a noncovalent interaction between an aromatic amino acid-enriched apoE N-terminal domain with the caveolin scaffolding domain. Absent apoE expression in adipocytes alters caveolar lipid composition. These observations provide evidence for an interaction between two proteins involved in cellular lipid metabolism in a cell specialized for lipid storage and flux, and rationalize a biological basis for the impact of adipocyte apoE expression on adipocyte lipoprotein metabolism.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cellular response to cholesterol  IEP 6904144 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Apoe  (apolipoprotein E)


Additional Information