RGD Reference Report - Cloning and crystal structure of hematopoietic prostaglandin D synthase. - Rat Genome Database

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Cloning and crystal structure of hematopoietic prostaglandin D synthase.

Authors: Kanaoka, Y  Ago, H  Inagaki, E  Nanayama, T  Miyano, M  Kikuno, R  Fujii, Y  Eguchi, N  Toh, H  Urade, Y  Hayaishi, O 
Citation: Kanaoka Y, etal., Cell 1997 Sep 19;90(6):1085-95.
RGD ID: 68838
Pubmed: PMID:9323136   (View Abstract at PubMed)

Hematopoietic prostaglandin (PG) D synthase is the key enzyme for production of the D and J series of prostanoids in the immune system and mast cells. We isolated a cDNA for the rat enzyme, crystallized the recombinant enzyme, and determined the three-dimensional structure of the enzyme complexed with glutathione at 2.3 A resolution. The enzyme is the first member of the sigma class glutathione S-transferase (GST) from vertebrates and possesses a prominent cleft as the active site, which is never seen among other members of the GST family. The unique 3-D architecture of the cleft leads to the putative substrate binding mode and its catalytic mechanism, responsible for the specific isomerization from PGH2 to PGD2.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
prostaglandin biosynthetic process  TAS 68838 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
prostaglandin-D synthase activity  TAS 68838 RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
prostanoid metabolic pathway  ISOHpgds (Rattus norvegicus)68838; 68838 RGD 
prostanoid metabolic pathway  IDA 68838 RGD 
Objects Annotated

Genes (Rattus norvegicus)
Hpgds  (hematopoietic prostaglandin D synthase)

Genes (Mus musculus)
Hpgds  (hematopoietic prostaglandin D synthase)

Genes (Homo sapiens)
HPGDS  (hematopoietic prostaglandin D synthase)


Additional Information