RGD Reference Report - Differential modulation of N-type 1B and P/Q-type 1A calcium channels by different G protein subunit isoforms. - Rat Genome Database

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Differential modulation of N-type 1B and P/Q-type 1A calcium channels by different G protein subunit isoforms.

Authors: Arnot, MI  Stotz, SC  Jarvis, SE  Zamponi, GW 
Citation: Arnot MI, etal., J Physiol 2000 Sep 1;527 Pt 2:203-12.
RGD ID: 68784
Pubmed: PMID:10970423   (View Abstract at PubMed)
PMCID: PMC2270070   (View Article at PubMed Central)

Using transient calcium phosphate transfection into the human embryonic kidney tsa-201 cell line and subsequent whole-cell patch-clamp protocols, we examined the tonic modulation of cloned N- and P/Q-type calcium channels by five different G protein beta subunits via strong depolarizing voltage prepulses. For N- and P/Q-type channels, the magnitude of inhibition was dependent on the Gbeta subtype co-expressed. Both the absolute and relative magnitudes of Gbeta subunit-induced inhibition of P/Q-type channels differed from those observed with the N-type channel. For each calcium channel subtype, kinetics of both the prepulse-mediated recovery from inhibition and the re-inhibition following the prepulse were examined for each of the Gbeta subunits by varying either the duration between the pre- and the test pulse or the length of the prepulse. For each channel subtype, we observed a differential Gbeta subunit rank order with regard to the rates of re-inhibition and recovery from inhibition. On average, P/Q-type channels exhibited more rapid rates of recovery from inhibition than those observed with N-type channels. Different Gbeta subtypes mediated different degrees of slowing of activation kinetics. The differential modulation of P/Q- and N-type channels by various Gbeta subtypes may provide a mechanism for fine tuning the amount of calcium entering the presynaptic nerve termini.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
calcium channel regulator activity  IMP 68784 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gnb2  (G protein subunit beta 2)


Additional Information