RGD Reference Report - Sequence, tissue distribution, and functional characterization of the rat fructose transporter GLUT5. - Rat Genome Database

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Sequence, tissue distribution, and functional characterization of the rat fructose transporter GLUT5.

Authors: Rand, EB  Depaoli, AM  Davidson, NO  Bell, GI  Burant, CF 
Citation: Rand EB, etal., Am J Physiol 1993 Jun;264(6 Pt 1):G1169-76.
RGD ID: 68309
Pubmed: PMID:8333543   (View Abstract at PubMed)

cDNA clones encoding rat GLUT5-small intestinal facilitative hexose transporter were isolated from a jejunum library by cross-hybridization with a human GLUT5 cDNA probe. The cDNA sequence indicates that rat GLUT5 is composed of 502 amino acids and has 81.5% amino acid identity and 87.3% similarity with the sequence of human GLUT5. Expression of synthetic rat GLUT5 mRNA in Xenopus oocytes showed that rat GLUT5 was able to mediate the uptake of fructose and, to a lesser extent, of glucose. RNA blotting studies showed that GLUT5 mRNA was present in rat small intestine, kidney, and brain. Although GLUT5 mRNA is expressed in human testis, adipose tissue, and skeletal muscle, it could not be detected by RNA blotting in these rat tissues. Developmental studies showed low levels of GLUT5 mRNA in rat small intestine and kidney during the prenatal period with a rapid induction of GLUT5 expression occurring postnatally. In situ hybridization studies of GLUT5 mRNA expression in the small intestine revealed differential expression along the crypt-villus axis with the highest levels of mRNA being in the midvillus region. In addition, there was quantitatively more GLUT5 mRNA detected in the proximal as opposed to the distal small intestine.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
fructose import across plasma membrane involved_inIDA 68309PMID:8333543UniProt 
fructose transmembrane transport  IDA 68309 RGD 
glucose transmembrane transport  IDA 68309 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
plasma membrane located_inIDA 68309PMID:8333543UniProt 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
fructose transmembrane transporter activity  IDA 68309 RGD 
glucose transmembrane transporter activity  IDA 68309 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc2a5  (solute carrier family 2 member 5)


Additional Information