RGD Reference Report - Study of retinoic acid effect upon retinoic acid receptors beta (RAR-beta) in C6 cultured glioma cells. - Rat Genome Database

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Study of retinoic acid effect upon retinoic acid receptors beta (RAR-beta) in C6 cultured glioma cells.

Authors: Reboul, P  George, P  Louisot, P  Broquet, P 
Citation: Reboul P, etal., Biochem Mol Biol Int. 1995 Aug;36(5):1097-105.
RGD ID: 6771324
Pubmed: PMID:7581005   (View Abstract at PubMed)

Using monoclonal antibodies against the RAR-alpha and RAR-beta retinoic receptors, we demonstrated that these receptors were present together in C6 glioma cells as two isoforms of 50 and 55 kDa. For RAR-beta, the 50 kDa isoform predominated (60 to 80% of the total of the two isoforms). After a treatment for 48 h with retinoic acid 10 microM, the 55 kDa form was enhanced, while no effect was observed either on RAR-alpha isoforms from C6 cells and on both RAR-alpha and RAR-beta forms from neuroblastoma SKN SH SY5Y used as a control. Using purified neuronal and glial rat brain nuclei, we showed that the 55 kDa isoform from RAR-beta predominated in glial cells. These results suggest that retinoic acid treatment of C6 cells led to a partial differentiation, the enhancement of the heavy form of RAR-beta being a marker of this phenomenon.

Objects referenced in this article
Gene Rara retinoic acid receptor, alpha Rattus norvegicus
Gene Rarb retinoic acid receptor, beta Rattus norvegicus

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