RGD Reference Report - The secretory phospholipase A2 gene is required for gastroesophageal reflux-related changes in murine esophagus. - Rat Genome Database

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The secretory phospholipase A2 gene is required for gastroesophageal reflux-related changes in murine esophagus.

Authors: Babu, A  Mauchley, D  Meng, X  Banerjee, AM  Gamboni-Robertson, F  Fullerton, DA  Weyant, MJ 
Citation: Babu A, etal., J Gastrointest Surg. 2009 Dec;13(12):2212-8. Epub 2009 Aug 12.
RGD ID: 6482726
Pubmed: PMID:19672667   (View Abstract at PubMed)
DOI: DOI:10.1007/s11605-009-0972-x   (Journal Full-text)

BACKGROUND: The initial response of esophageal mucosa to gastroduodenal reflux is inflammation and hyperplasia. Secretory phospholipase A(2) (sPLA(2)) is a known mediator of gut inflammation, and its levels are increased in Barrett's esophagus. We hypothesized that the sPLA(2) gene is required to produce esophageal mucosal hyperplasia in response to gastroduodenal reflux. METHODS: C57BL/6 (n = 5) sPLA(2) (-/-) mice and C57BL/6( Cg-Tg(PLA2G2A)703N16 ) mice (n = 4) sPLA(2) (-/+) underwent a side-to-side surgical anastomosis between the duodenum and gastroesophageal junction (DGEA). Control animals [sPLA(2) (-/-) (n = 5), sPLA(2) (-/+) (n = 4)] underwent laparotomy with incision and repair of the esophagus. Tissue was harvested after 4 weeks, and H&E staining was performed to quantify esophageal mucosal thickness. Ki67 and sPLA(2) immunostaining were performed to quantitate differences in cell division and sPLA(2) expression. RESULTS: Mice expressing human sPLA(2) had a 2.5-fold increase in thickness of the esophageal mucosa as compared to controls (p = 0.01). A 6.5-fold increase in proliferation (p = 0.02) and a twofold increase in sPLA(2) expression (p = 0.04) were demonstrated in animals exposed to gastroduodenal reflux. CONCLUSIONS: The presence of sPLA(2) is necessary for early mucosal hyperplasia produced by exposure of the esophagus to gastroduodenal contents. sPLA(2) expression is upregulated by gastroduodenal reflux, strengthening its role as a critical mediator of early mucosal hyperplasia.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
gastroesophageal reflux disease  IMP 6482726 RGD 
gastroesophageal reflux disease  ISOPLA2G2A (Homo sapiens)6482726; 6482726 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Pla2g2a  (phospholipase A2 group IIA)

Genes (Mus musculus)
Pla2g2a  (phospholipase A2, group IIA (platelets, synovial fluid))

Genes (Homo sapiens)
PLA2G2A  (phospholipase A2 group IIA)


Additional Information