RGD Reference Report - The putative rat choline transporter CHOT1 transports creatine and is highly expressed in neural and muscle-rich tissues. - Rat Genome Database

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The putative rat choline transporter CHOT1 transports creatine and is highly expressed in neural and muscle-rich tissues.

Authors: Schloss, P  Mayser, W  Betz, H 
Citation: Schloss P, etal., Biochem Biophys Res Commun 1994 Jan 28;198(2):637-45.
RGD ID: 634748
Pubmed: PMID:8297374   (View Abstract at PubMed)
DOI: DOI:10.1006/bbrc.1994.1093   (Journal Full-text)

A rabbit homolog of the putative rat choline transporter CHOT1 has recently been shown to mediate creatine transport (Guimbal and Kilimann, J. Biol. Chem., 1993). Here, the functional properties of the CHOT1 cDNA were studied by heterologous expression in HEK-293 cells. After transfection, the cells displayed a 7-8fold stimulation of [14C]creatine uptake with a Km of 46.2 microM. This transport was inhibited by the creatine uptake inhibitor beta-guanidinopropionate (Ki of 23 microM). Northern blot analysis revealed a major transcript of 4.8 kb in brain, heart, skeletal muscle and kidney. In situ hybridization showed high transcript levels in the cerebellum, hippocampus and other brain regions. High expression was also seen in the rat embryo along the entire neuraxis and in some non-neuronal tissues. These data establish CHOT1 as a widely expressed rat creatine transporter.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
creatine transmembrane transport  IDA 634748 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
choline transmembrane transporter activity  NAS 634748 RGD 
creatine transmembrane transporter activity  IDA 634748 RGD 
creatine:sodium symporter activity enablesIDA 634748PMID:8297374UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Slc6a8  (solute carrier family 6 member 8)


Additional Information