RGD Reference Report - Effect of long-term exercise on gene expression of insulin signaling pathway intermediates in skeletal muscle. - Rat Genome Database

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Effect of long-term exercise on gene expression of insulin signaling pathway intermediates in skeletal muscle.

Authors: Kim, YB  Inoue, T  Nakajima, R  Shirai-Morishita, Y  Tokuyama, K  Suzuki, M 
Citation: Kim YB, etal., Biochem Biophys Res Commun 1999 Jan 27;254(3):720-7.
RGD ID: 633968
Pubmed: PMID:9920808   (View Abstract at PubMed)
DOI: DOI:10.1006/bbrc.1998.9940   (Journal Full-text)

To elucidate the molecular mechanism underlying insulin sensitivity, we have thought to investigate gene expression of insulin signaling pathway intermediates in skeletal muscle from sedentary and endurance-trained rats. Adult male Sprague-Dawley rats were trained for 9 weeks on a treadmill; 30 m/min at a 6 degrees incline, 90 min/day, 5 days/week. The levels of PI 3-kinase, GLUT4, p70 S6 kinase and Ras mRNA were significantly increased by 89, 40, 38, and 47%, respectively, with running training; however, the Nck mRNA level was decreased by 24%. mRNA levels of SHP-2, Grb2, Sos, Shc, GAP, p62 and p90 S6 kinase were unaltered by running training. We have previously reported that endurance training increases mRNA levels of insulin receptor, IRS-1 and ERK1 in skeletal muscle of rats. Taken together, our data suggest that gene expression of the insulin signal pathway intermediates is modulated by endurance training that may be associated with alteration of insulin sensitivity.

Objects referenced in this article
Gene Khdrbs1 KH RNA binding domain containing, signal transduction associated 1 Rattus norvegicus
Gene Ptpn11 protein tyrosine phosphatase, non-receptor type 11 Rattus norvegicus
Gene Rps6ka2 ribosomal protein S6 kinase A2 Rattus norvegicus
Gene Shc1 SHC adaptor protein 1 Rattus norvegicus
Gene Sos2 SOS Ras/Rho guanine nucleotide exchange factor 2 Rattus norvegicus

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