RGD Reference Report - Purification and characterization of a naturally occurring vascular endothelial growth factor.placenta growth factor heterodimer. - Rat Genome Database

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Purification and characterization of a naturally occurring vascular endothelial growth factor.placenta growth factor heterodimer.

Authors: DiSalvo, J  Bayne, ML  Conn, G  Kwok, PW  Trivedi, PG  Soderman, DD  Palisi, TM  Sullivan, KA  Thomas, KA 
Citation: DiSalvo J, etal., J Biol Chem 1995 Mar 31;270(13):7717-23.
RGD ID: 633657
Pubmed: PMID:7706320   (View Abstract at PubMed)

Vascular endothelial growth factor (VEGF) is a potent and selective mitogen for endothelial cells that is angiogenic in vivo and induced by hypoxia. A homologous protein, placenta growth factor (PlGF), is also reported to be mitogenic for endothelial cells in culture. The rat GS-9L glioma cell line produces not only VEGF homodimers but also PlGF homodimers and a novel heterodimer composed of VEGF and PlGF subunits. All three dimeric forms were purified to apparent homogeneity, and their structures and mitogenic activities were compared. VEGF.PlGF heterodimers are vascular endothelial cell mitogens nearly as potent as VEGF homodimers. Therefore, some of the biological activities attributed to VEGF homodimers might be mediated by VEGF.PlGF heterodimers. In contrast, pure PlGF homodimers are mitogenic for endothelial cells only at high, possibly non-physiologic concentrations; thus the biological relevance of their mitogenic activity for these cells is not obvious. However, the existence of not only homodimers but also heterodimers clearly extends the similarity between the VEGF/PlGF and the homologous platelet-derived growth factor systems.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
identical protein binding  IPIPgf (Rattus norvegicus)633657homodimerizationRGD 
protein-containing complex binding  IDA 633657heterodimerizationRGD 
vascular endothelial growth factor receptor binding  TAS 633657 RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
vascular endothelial growth factor signaling pathway   TAS 633657 RGD 
Objects Annotated

Genes (Rattus norvegicus)
Pgf  (placental growth factor)


Additional Information