RGD Reference Report - A cytosolic, galphaq- and betagamma-insensitive splice variant of phospholipase C-beta4. - Rat Genome Database

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A cytosolic, galphaq- and betagamma-insensitive splice variant of phospholipase C-beta4.

Authors: Kim, MJ  Min, DS  Ryu, SH  Suh, PG 
Citation: Kim MJ, etal., J Biol Chem 1998 Feb 6;273(6):3618-24.
RGD ID: 633576
Pubmed: PMID:9452490   (View Abstract at PubMed)

Phospholipase C (PLC)-beta4 has been considered to be a mammalian homolog of the NorpA PLC, which is responsible for visual signal transduction in Drosophila. We reported previously the cloning of a cDNA encoding rat phospholipase C-beta4 (PLC-beta4) (Kim, M. J., Bahk, Y. Y., Min, D. S., Lee, S. J., Ryu, S. H., and Suh, P.-G. (1993) Biochem. Biophys. Res. Commun. 194, 706-712). We report now the isolation and characterization of a splice variant (PLC-beta4b). PLC-beta4b is identical to the 130-kDa PLC-beta4 (PLC-beta4a) except that the carboxyl-terminal 162 amino acids of PLC-beta4a are replaced by 10 distinct amino acids. The existence of PLC-beta4b transcripts in the rat brain was demonstrated by reverse transcription-polymerase chain reaction analysis. Immunological analysis using polyclonal antibody specific for PLC-beta4b revealed that this splice variant exists in rat brain cytosol. To investigate functional differences between the two forms of PLC-beta4, transient expression studies in COS-7 cells were conducted. We found that PLC-beta4a was localized mainly in the particulate fraction of the cell, and it could be activated by Galphaq, whereas PLC-beta4b was localized exclusively in the soluble fraction, and it could not be activated by Galphaq. In addition, both PLC-beta4a and PLC-beta4b were not activated by G-protein betagamma-subunits purified from rat brain. These results suggest that PLC-beta4b may be regulated by a mechanism different from that of PLC-beta4a, and therefore it may play a distinct role in PLC-mediated signal transduction.

Objects referenced in this article
Gene Plcb4 phospholipase C, beta 4 Rattus norvegicus
Gene Plcb4_v2 phospholipase C, beta 4, variant 2 Rattus norvegicus

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