RGD Reference Report - Cloning of human muscle phosphofructokinase cDNA. - Rat Genome Database

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Cloning of human muscle phosphofructokinase cDNA.

Authors: Nakajima, H  Noguchi, T  Yamasaki, T  Kono, N  Tanaka, T  Tarui, S 
Citation: Nakajima H, etal., FEBS Lett 1987 Oct 19;223(1):113-6.
RGD ID: 633571
Pubmed: PMID:2822475   (View Abstract at PubMed)

Three overlapping cDNA clones for human muscle phosphofructokinase (HMPFK) covering the complete coding sequence were isolated. The sequence included a poly(A) tail, a 399 bp 3'-untranslated region, a 2337 bp coding region for 779 amino acid residues and a part of the 5'-untranslated region. Homologies between HMPFK and rabbit muscle phosphofructokinase (RMPFK) were 96% of the amino acids and 89% of the nucleotides in the coding region. Like RMPFK, HMPFK also possessed the internal homology between C- and N-halves in its primary structure. Cloning of HMPFK cDNA will help to identify the molecular defect in patients with glycogenosis type VII (HMPFK deficiency).

Objects referenced in this article
Gene Pfkm phosphofructokinase, muscle Rattus norvegicus

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