RGD Reference Report - Involvement of epidermal growth factor-like domain of NELL proteins in the novel protein-protein interaction with protein kinase C. - Rat Genome Database

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Involvement of epidermal growth factor-like domain of NELL proteins in the novel protein-protein interaction with protein kinase C.

Authors: Kuroda, S  Tanizawa, K 
Citation: Kuroda S and Tanizawa K, Biochem Biophys Res Commun 1999 Nov 30;265(3):752-7.
RGD ID: 633406
Pubmed: PMID:10600492   (View Abstract at PubMed)
DOI: DOI:10.1006/bbrc.1999.1753   (Journal Full-text)

NELL proteins are the thrombospondin-1-like proteins that are strongly expressed in neural tissues, containing six epidermal growth factor (EGF)-like domains. By radiolabeling of the NELL protein-expressing COS-7 cells with [(32)P]orthophosphate, here we demonstrate that NELL proteins are synthesized as phosphoproteins by interacting with protein kinases in the cells. By immunoprecipitation and in vitro phosphorylation assays, we have also found that NELL proteins expressed in COS-7 cells are associated with and phosphorylated by protein kinase C betaI (PKCbetaI). Further analysis using various deletion mutants of NELL proteins by the yeast two-hybrid assay has revealed their EGF-like domains to be involved in the isoform-specific interaction with PKC. Conversely, the NH(2)-terminal variable region of PKC isoforms has been found essential for the interaction with NELL proteins. Because NELL proteins are expressed mainly in the cytoplasm of neuronal cells, unlike most EGF-like domain-containing extracellular proteins, the novel protein-protein interaction identified here between the EGF-like domains of NELL proteins and PKC suggests that EGF-like domains of intracellular proteins can be a target of PKC that mediates various signaling pathways.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
protein kinase C binding  IDA 633406; 633406 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Nell1  (neural EGFL like 1)
Nell2  (neural EGFL like 2)


Additional Information