RGD Reference Report - Identification of a short form of the P2xR1-purinoceptor subunit produced by alternative splicing in the pituitary and cochlea. - Rat Genome Database

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Identification of a short form of the P2xR1-purinoceptor subunit produced by alternative splicing in the pituitary and cochlea.

Authors: Housley, GD  Greenwood, D  Bennett, T  Ryan, AF 
Citation: Housley GD, etal., Biochem Biophys Res Commun 1995 Jul 17;212(2):501-8.
RGD ID: 633339
Pubmed: PMID:7542879   (View Abstract at PubMed)
DOI: DOI:10.1006/bbrc.1995.1998   (Journal Full-text)

A truncated form of the P2xR1 purinoceptor subunit (which we designate P2xR1-2) was detected in rat pituitary gland and the secretory epithelial tissue (stria vascularis) of the cochlea using RT-PCR of solid-phase cDNA libraries. PCR products corresponding to the P2xR1 purinoceptor subunit (1) were obtained from vas deferens, brain and microdissected cochlear sensory epithelial tissues including organ of Corti, sacculus and crista ampullaris. Cloning and sequencing revealed that the P2xR1-2 product included an 85-bp insertion in a region corresponding to a novel C-terminal end of the second membrane spanning domain and continuing as the cytoplasmic domain. A stop codon sequence after the first 51 bp of the insert effectively truncates this subunit, reducing the final cytoplasmic domain by 90% compared with the previously published P2xR1(-1) sequence, thereby reducing the overall peptide by approximately 25%. The region of the receptor lost in the truncated version coded for a number of serine/proline rich regions which may act as potential intracellular regulatory sites.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
ligand-gated monoatomic ion channel activity  IDA 633339 RGD 

Objects Annotated

Genes (Rattus norvegicus)
P2rx2  (purinergic receptor P2X 2)


Additional Information