RGD Reference Report - Ly-49s3 is a promiscuous activating rat NK cell receptor for nonclassical MHC class I-encoded target ligands. - Rat Genome Database

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Ly-49s3 is a promiscuous activating rat NK cell receptor for nonclassical MHC class I-encoded target ligands.

Authors: Naper, C  Hayashi, S  Kveberg, L  Niemi, EC  Lanier, LL  Vaage, JT  Ryan, JC 
Citation: Naper C, etal., J Immunol 2002 Jul 1;169(1):22-30.
RGD ID: 633264
Pubmed: PMID:12077224   (View Abstract at PubMed)

Previous studies of the rapid rejection of MHC-disparate lymphocytes in rats, named allogeneic lymphocyte cytotoxicity, have indicated that rat NK cells express activating receptors for nonclassical MHC class I allodeterminants from the RT1-C/E/M region. Using an expression cloning system that identifies activating receptors associated with the transmembrane adapter molecule DAP12, we have cloned a novel rat Ly-49 receptor that we have termed Ly-49 stimulatory receptor 3 (Ly-49s3). A newly generated anti-Ly-49s3 Ab, mAb DAR13, identified subpopulations of resting and IL-2-activated NK cells, but not T or B lymphocytes. Depletion of Ly-49s3-expressing NK cells drastically reduced alloreactivity in vitro, indicating that this subpopulation is responsible for a major part of the observed NK alloreactivity. DAR13-mediated blockade of Ly-49s3 inhibited killing of MHC-congenic target cells from the av1, n, lv1, and c haplotypes, but not from the u or b haplotypes. A putative ligand was mapped to the nonclassical MHC class I region (RT1-C/E/M) using intra-MHC recombinant strains. Relative numbers of Ly-49s3(+) NK cells were reduced, and surface levels of Ly-49s3 were lower, in MHC congenic strains expressing the putative Ly-49s3 ligand(s). In conclusion, we have identified a novel Ly-49 receptor that triggers rat NK cell-mediated responses.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of natural killer cell mediated cytotoxicity  IDA 633264 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cell surface  IDA 633264 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
identical protein binding  IPILy49s3 (Rattus norvegicus)633264homodimerizationRGD 
protein binding  IPITyrobp (Rattus norvegicus)633264 RGD 
protein binding  IPILy49s3 (Rattus norvegicus)633264 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ly49s3  (Ly-49 stimulatory receptor 3)
Tyrobp  (transmembrane immune signaling adaptor Tyrobp)

Objects referenced in this article
Gene Klra22 killer cell lectin-like receptor subfamily A, member 22 Rattus norvegicus

Additional Information