RGD Reference Report - Gene expression of rat glutathione S-transferases. Evidence for gene conversion in the evolution of the Yb multigene family. - Rat Genome Database

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Gene expression of rat glutathione S-transferases. Evidence for gene conversion in the evolution of the Yb multigene family.

Authors: Lai, HC  Qian, B  Grove, G  Tu, CP 
Citation: Lai HC, etal., J Biol Chem 1988 Aug 15;263(23):11389-95.
RGD ID: 632976
Pubmed: PMID:3403534   (View Abstract at PubMed)

We have characterized a cDNA with complete coding sequence for the rat liver glutathione S-transferase subunit 4 (Yb2) isolated from a constructed lambda gt10 cDNA library. Functional expression of the cDNA sequence has resulted in the purification to homogeneity of an enzymatically active anionic glutathione S-transferase. In addition to three previously described Yb-type subunits (Yb1, Yb2, Yb3), we now report characterization of a fourth Yb subunit sequence in the form of a genomic DNA clone lambda GTR15-2. The Yb4 gene has no apparent defect, and the deduced Yb4 polypeptide sequence differs from the other three Ybs by 40 to 53 amino acids. The Yb4 gene organization is similar to that of the Yb2 gene in having a minimum of eight exons. Three out of the seven introns between the two genes are conserved to the extent of more than 88% nucleotide identity. We propose that gene conversion may have played a role in the evolution of these Yb genes.

Objects referenced in this article
Gene Gstm2 glutathione S-transferase mu 2 Rattus norvegicus
Gene Gstm3 glutathione S-transferase mu 3 Rattus norvegicus

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