RGD Reference Report - Molecular cloning and sequence determination of cDNAs for alpha subunits of the guanine nucleotide-binding proteins Gs, Gi, and Go from rat brain. - Rat Genome Database

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Molecular cloning and sequence determination of cDNAs for alpha subunits of the guanine nucleotide-binding proteins Gs, Gi, and Go from rat brain.

Authors: Itoh, H  Kozasa, T  Nagata, S  Nakamura, S  Katada, T  Ui, M  Iwai, S  Ohtsuka, E  Kawasaki, H  Suzuki, K 
Citation: Itoh H, etal., Proc Natl Acad Sci U S A 1986 Jun;83(11):3776-80.
RGD ID: 632849
Pubmed: PMID:3086867   (View Abstract at PubMed)
PMCID: PMC323606   (View Article at PubMed Central)

We have cloned cDNAs encoding alpha subunits of the guanine nucleotide-binding proteins Gs, Gi, and Go and determined their nucleotide sequences. Purified preparations of Gi and Go alpha subunits (Gi alpha and Go alpha) from rat brain were completely digested with trypsin, and peptides were subjected to amino acid sequence analysis. By screening of a cDNA library from rat C6 glioma cells with a synthetic probe corresponding to a 17 amino acid sequence, a clone encoding the sequence of Go alpha was obtained. Then, the library was rescreened with a Go alpha cDNA probe to isolate several strongly or weakly hybridizing clones. cDNAs encoding the complete sequences of Gi alpha and Gs alpha were thus obtained. From nucleotide sequence analysis, the amino acid sequences of Gs alpha and Gi alpha were deduced; they contain 394 and 355 amino acid residues (including the initiator methionine), respectively. The calculated molecular weights for Gs alpha and Gi alpha were 45,663 and 40,499, respectively. The Go alpha clone encoded a sequence of 310 amino acid residues that lacked the NH2 terminus. The homology of the alpha subunits of Gs, Gi, Go, transducin, and ras-encoded protein is discussed.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
GTP binding  TAS 632849 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gnas  (GNAS complex locus)

Objects referenced in this article
Gene Gnao1 guanine nucleotide binding protein, alpha O Mus musculus
Gene Gnai2 G protein subunit alpha i2 Rattus norvegicus
Gene Gnao1 G protein subunit alpha o1 Rattus norvegicus

Additional Information