RGD Reference Report - Molecular cloning of five GTP-binding protein cDNA species from rat olfactory neuroepithelium. - Rat Genome Database

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Molecular cloning of five GTP-binding protein cDNA species from rat olfactory neuroepithelium.

Authors: Jones, DT  Reed, RR 
Citation: Jones DT and Reed RR, J Biol Chem 1987 Oct 15;262(29):14241-9.
RGD ID: 632848
Pubmed: PMID:2820999   (View Abstract at PubMed)

Biochemical studies in vertebrate olfactory tissue indicate that certain odorants stimulate adenylyl cyclase in a GTP-dependent manner. Additionally, immunochemical and toxin-labeling studies demonstrate the presence of several GTP-binding protein (G-protein) species in vertebrate olfactory epithelium. To identify the G-protein(s) responsible for olfactory signal transduction, we screened a rat olfactory cDNA library with an oligonucleotide probe and isolated 32 recombinant clones encoding five distinct types of G-protein alpha subunits. The majority of the clones encoded G alpha s, while the remaining clones encoded G alpha o, G alpha i1, G alpha i2, and a novel species, G alpha i3. Messenger RNA corresponding to each G alpha was detectable in all tissues examined; however, the levels for a given G alpha varied in a tissue-specific manner. In olfactory tissue, G alpha s was the most abundant of these messages and in combination with the biochemical studies suggests that G alpha s is the G-protein component of the olfactory signal transduction cascade.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
GDP binding  TAS 632848 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Gnao1  (G protein subunit alpha o1)

Objects referenced in this article
Gene Gnai1 G protein subunit alpha i1 Mus musculus
Gene Gnai3 G protein subunit alpha i3 Mus musculus
Gene Gnai1 G protein subunit alpha i1 Rattus norvegicus
Gene Gnai2 G protein subunit alpha i2 Rattus norvegicus
Gene Gnai3 G protein subunit alpha i3 Rattus norvegicus
Gene Gnas GNAS complex locus Rattus norvegicus

Additional Information