RGD Reference Report - Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family. - Rat Genome Database

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Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family.

Authors: Fort, P  Marty, L  Piechaczyk, M  El Sabrouty, S  Dani, C  Jeanteur, P  Blanchard, JM 
Citation: Fort P, etal., Nucleic Acids Res 1985 Mar 11;13(5):1431-42.
RGD ID: 632774
Pubmed: PMID:2987824   (View Abstract at PubMed)
PMCID: PMC341087   (View Article at PubMed Central)

We have isolated and sequenced a full-length cDNA clone encoding rat glyceraldehyde-3-phosphate-dehydrogenase (GAPDH, E.C.1.2.1.12). The entire mRNA is 1269 nucleotides long exclusive of poly(A) and contains respectively 71 and 196 bases of 5' and 3' non-coding regions. Primer extension as well as S1 nuclease protection experiments clearly established that a single (or at least a highly prominent) GAPDH mRNA species is expressed in all rat tissues examined. This sequence allowed the determination of the hitherto unknown primary structure of rat GAPDH which is 333 aminoacids long. Comparison between GAPDH sequences from rat, man and chicken revealed a high degree of sequence conservation at both nucleotide and protein levels.

Objects referenced in this article
Gene Gapdh glyceraldehyde-3-phosphate dehydrogenase Rattus norvegicus

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