RGD Reference Report - Identification and molecular evolution of new dynein-like protein sequences in rat brain. - Rat Genome Database

RGD Reference Report - Identification and molecular evolution of new dynein-like protein sequences in rat brain. - Rat Genome Database

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Identification and molecular evolution of new dynein-like protein sequences in rat brain.
Authors:	Tanaka, Y Zhang, Z Hirokawa, N
Citation:	Tanaka Y, etal., J Cell Sci 1995 May;108 ( Pt 5):1883-93.
RGD ID:	632520
Pubmed:	PMID:7657712 (View Abstract at PubMed)
RT-PCR cloning was performed to find unknown members of the dynein superfamily expressed in rat brain. Six kinds of degenerate primers designed for the dynein catalytic domain consensuses were used for extensive PCR amplifications. We have sequenced 550 plasmid clones which turned out to include 13 kinds of new dynein-like sequences (DLP1-8, 9A/B, 10-12) and cytoplasmic dynein heavy chain. In these clones, alternative splicing was detected for a 105 nt-domain containing the CFDEFNRI consensus just downstream of the most N-terminal P-loop (DLP9A and 9B). By using these obtained sequences, initial hybridization studies were performed. Genomic Southern blotting showed each sequence corresponds to a single copy of the gene, while northern blotting of adult brain presented more than one band for some subtypes. We further accomplished molecular evolutionary analysis to recognize their phylogenetic origins for the axonemal and non-axonemal (cytoplasmic) functions. Different methods (UPGMA, NJ and MP) presented well coincident phylogenetic trees from 44 partial amino acid sequences of dynein heavy chain from various eukaryotes. The trunk for all the cytoplasmic dynein heavy chain homologues diverged directly from the root of the phylogenetic tree, suggesting that the first dynein gene duplication defined two distinct functions as respective subfamilies. Of particular interest, we found a duplication event of the cytoplasmic dynein heavy chain gene giving rise to another subtype, DLP4, located between the divergence of yeast and that of Dictyostelium. Such evolutionary topology builds up an inceptive hypothesis that there are at least two non-axonemal dynein heavy chains in mammals.

Objects referenced in this article

Gene	Dnah1	dynein, axonemal, heavy chain 1	Rattus norvegicus
Gene	Dnah10	dynein, axonemal, heavy chain 10	Rattus norvegicus
Gene	Dnah11	dynein, axonemal, heavy chain 11	Rattus norvegicus
Gene	Dnah2	dynein, axonemal, heavy chain 2	Rattus norvegicus
Gene	Dnah5	dynein, axonemal, heavy chain 5	Rattus norvegicus
Gene	Dnah6	dynein, axonemal, heavy chain 6	Rattus norvegicus
Gene	Dnah7	dynein, axonemal, heavy chain 7	Rattus norvegicus
Gene	Dnah8	dynein, axonemal, heavy chain 8	Rattus norvegicus
Gene	Dnah9	dynein, axonemal, heavy chain 9	Rattus norvegicus
Gene	Dync2h1	dynein cytoplasmic 2 heavy chain 1	Rattus norvegicus

Additional Information