RGD Reference Report - Expression of the AMP-activated protein kinase beta1 and beta2 subunits in skeletal muscle. - Rat Genome Database

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Expression of the AMP-activated protein kinase beta1 and beta2 subunits in skeletal muscle.

Authors: Chen, Z  Heierhorst, J  Mann, RJ  Mitchelhill, KI  Michell, BJ  Witters, LA  Lynch, GS  Kemp, BE  Stapleton, D 
Citation: Chen Z, etal., FEBS Lett 1999 Oct 29;460(2):343-8.
RGD ID: 631899
Pubmed: PMID:10544261   (View Abstract at PubMed)

A heterotrimeric member of the AMP-activated protein kinase (AMPK) isoenzyme family was purified from rat skeletal muscle by immunoaffinity chromatography, consisting of an alpha2 catalytic and two non-catalytic subunits, beta2 and gamma1. The AMPK beta2 cDNA (271 amino acids (aa), molecular weight (MW)=30 inverted question mark omitted inverted question mark307, pI 6. 3) was cloned from skeletal muscle and found to share an overall identity of 70% with beta1 (270 aa, MW=30 inverted question mark omitted inverted question mark475, pI 6.0). In the liver AMPK beta1 subunit, Ser-182 is constitutively phosphorylated whereas in skeletal muscle beta2 isoform, we find that Ser-182 is only partially phosphorylated. In addition, the autophosphorylation sites Ser-24, Ser-25 found in the beta1 are replaced by Ala-Glu in the beta2 isoform. beta2 contains seven more Ser and one less Thr residues than beta1, raising the possibility of differential post-translational regulation. Immunoblot analysis further revealed that soleus muscle (slow twitch) contains exclusively beta1 associated with alpha2, whereas extensor digitorum longus muscle alpha2 (EDL, fast twitch) associates with beta2 as well as beta1. Sequence analysis revealed that glycogen synthase, a known AMPK substrate, co-immunoprecipitated with the AMPK alpha2beta2gamma1 complex.

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
cAMP-dependent protein kinase complex  IDA 631899 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Prkab2  (protein kinase AMP-activated non-catalytic subunit beta 2)

Objects referenced in this article
Gene Prkab1 protein kinase, AMP-activated, beta 1 non-catalytic subunit Mus musculus
Gene Prkab2 protein kinase, AMP-activated, beta 2 non-catalytic subunit Mus musculus

Additional Information