RGD Reference Report - Astrocytic but not neuronal increased expression and redistribution of parkin during unfolded protein stress. - Rat Genome Database

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Astrocytic but not neuronal increased expression and redistribution of parkin during unfolded protein stress.

Authors: Ledesma, MD  Galvan, C  Hellias, B  Dotti, C  Jensen, PH 
Citation: Ledesma MD, etal., J Neurochem 2002 Dec;83(6):1431-40.
RGD ID: 628414
Pubmed: PMID:12472897   (View Abstract at PubMed)

Parkin is a ubiquitin ligase that facilitates proteasomal protein degradation and is involved in a common autosomal recessive form of Parkinson's disease. Its expression is part of the unfolded protein response in cell lines where its overexpression protects against unfolded protein stress. How parkin expression is regulated in brain primary cells under stress situations is however, less well established. Here, the cellular and subcellular localization of parkin under basal conditions and during unfolded protein stress was investigated in primary cultures of rat astrocytes and hippocampal neurons. Immunofluorescense microscopy and biochemical analysis demonstrated that parkin is mainly associated with the endoplasmic reticulum (ER) in hippocampal neurons while it is associated with Golgi membranes, the nuclei and light vesicles in astrocytes. The constitutive parkin expression was high in neurons as compared with astrocytes. However, unfolded protein stress elicited a selective increase in astrocytic parkin expression and a change in distribution, whereas neuronal parkin remained largely unmodified. The cell specific differences argue in favour of different cellular binding sites and substrates for the protein and a pathogenic role for astrocytes in Parkinson's disease caused by parkin dysfunction.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
response to unfolded protein  IEP 628414 RGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Golgi membrane  IDA 628414 RGD 
intracellular vesicle  IDA 628414 RGD 
neuronal cell body  IDA 628414 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Prkn  (parkin RBR E3 ubiquitin protein ligase)


Additional Information