RGD Reference Report - Ribozyme compromise of adrenomedullin mRNA reveals a physiological role in the regulation of water intake. - Rat Genome Database

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Ribozyme compromise of adrenomedullin mRNA reveals a physiological role in the regulation of water intake.

Authors: Taylor, MM  Samson, WK 
Citation: Taylor MM and Samson WK, Am J Physiol Regul Integr Comp Physiol 2002 Jun;282(6):R1739-45.
RGD ID: 628326
Pubmed: PMID:12010756   (View Abstract at PubMed)
DOI: DOI:10.1152/ajpregu.00696.2001   (Journal Full-text)

The adrenomedullin (AM) preprohormone is posttranslationally processed to result in two biologically active fragments, AM and proadrenomedullin NH(2)-terminal 20 peptide (PAMP). AM is thought to play a role in fluid and electrolyte balance by acting in brain to inhibit salt and water appetite and in the kidney to cause diuresis and natriuresis. We previously have shown that AM is necessary for the short-term regulation of salt intake. In this paper, we have designed a ribozyme, a catalytic RNA molecule, which specifically recognizes and cleaves the AM transcript. In cultured vascular smooth muscle cells, ribozyme treatment lowered AM mRNA and reduced peptide content. Intracerebroventricular administration of the ribozyme lowered hypothalamic AM content and led to an exaggerated drinking response in rats, demonstrating that endogenous, brain-derived AM is physiologically relevant and necessary for short-term control of water intake.

Objects referenced in this article
Gene Adm adrenomedullin Rattus norvegicus

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