RGD Reference Report - Molecular evidence for a glycine-gated chloride channel in macrophages and leukocytes. - Rat Genome Database

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Molecular evidence for a glycine-gated chloride channel in macrophages and leukocytes.

Authors: Froh, M  Thurman, RG  Wheeler, MD 
Citation: Froh M, etal., Am J Physiol Gastrointest Liver Physiol 2002 Oct;283(4):G856-63.
RGD ID: 625689
Pubmed: PMID:12223345   (View Abstract at PubMed)
DOI: DOI:10.1152/ajpgi.00503.2001   (Journal Full-text)

Recent studies have demonstrated that glycine blunts the response of Kupffer cells to endotoxin. Based on pharmacological evidence, it was hypothesized that Kupffer cells and other macrophages contain a glycine-gated chloride channel similar to the glycine receptor expressed in neuronal tissues. Moreover, glycine stimulates influx of radiolabeled chloride in Kupffer cells in a dose-dependent manner. RT-PCR was used to identify mRNA of both alpha- and beta-subunits of the glycine receptor in rat Kupffer cells, peritoneal neutrophils, and splenic and alveolar macrophages, similar to the sequence generated from rat spinal cord. Importantly, the sequence of the cloned Kupffer cell glycine receptor fragment for the beta-subunit was >95% homologous with the receptor from the spinal cord. Membranes of these cells also contain a protein that is immunoreactive with antibodies against the glycine-gated chloride channel. These data demonstrate that Kupffer cells, as well as other macrophages and leukocytes, express mRNA and protein for a glycine-gated chloride channel with both molecular and pharmacological properties similar to the channel expressed in the central nervous system.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
monoatomic anion transport  IDA 625689 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
extracellularly glycine-gated chloride channel activity  IDA 625689 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Glra1  (glycine receptor, alpha 1)


Additional Information