RGD Reference Report - Characterization of a novel isoform of caspase-9 that inhibits apoptosis. - Rat Genome Database

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Characterization of a novel isoform of caspase-9 that inhibits apoptosis.

Authors: Angelastro, JM  Moon, NY  Liu, DX  Yang, AS  Greene, LA  Franke, TF 
Citation: Angelastro JM, etal., J Biol Chem 2001 Apr 13;276(15):12190-200.
RGD ID: 62424
Web Url: http://www.jbc.org/cgi/content/full/276/15/12190
Pubmed: PMID:11278518   (View Abstract at PubMed)
DOI: DOI:10.1074/jbc.M009523200   (Journal Full-text)

We have identified a novel isoform of rat caspase-9 in which the C terminus of full-length caspase-9 is replaced with an alternative peptide sequence. Casp-9-CTD (where CTD is carboxyl-terminal divergent) is expressed in multiple tissues, with the relative highest expression observed in ovary and heart. Casp-9-CTD was found primarily in the cytoplasm and was not detected in the nucleus. Structural predictions suggest that in contrast to full-length caspase-9, casp-9-CTD will not be processed. Our model is supported by reduced protease activity of casp-9-CTD preparations in vitro and by the lack of detectable processing of casp-9-CTD proenzyme or the induction of cell death following transfection into cells. Both neuronal and non-neuronal cell types transfected with casp-9-CTD were resistant to death evoked by trophic factor deprivation or DNA damage. In addition, cytosolic lysates prepared from cells permanently expressing exogenous casp-9-CTD were resistant to caspase induction by cytochrome c in reconstitution assays. Taken together, our observations indicate that casp-9-CTD acts as a dominant-negative variant. Its expression in various tissues indicates a physiological role in regulating cell death.

Objects referenced in this article
Gene Casp9 caspase 9 Rattus norvegicus
Gene Casp9_v1 caspase-9-carboxyl-terminal divergent Rattus norvegicus

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