RGD Reference Report - Spacing and orientation of bipartite DNA-binding motifs as potential functional determinants for POU domain factors. - Rat Genome Database

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Spacing and orientation of bipartite DNA-binding motifs as potential functional determinants for POU domain factors.

Authors: Li, P  He, X  Gerrero, MR  Mok, M  Aggarwal, A  Rosenfeld, MG 
Citation: Li P, etal., Genes Dev 1993 Dec;7(12B):2483-96.
RGD ID: 61700
Pubmed: PMID:8276233   (View Abstract at PubMed)

Investigation of the large POU domain family of developmental regulators has revealed a molecular mechanism by which highly related transcription factors sharing common DNA-binding motifs act to functionally discriminate their cognate DNA sequences. Studies of two classes of neuron-specific POU domain factors (III and IV) indicate that functional specificity on their native response elements is achieved by accommodating different nucleotide spacing between variably oriented bipartite core DNA-binding motifs. The preferred orientation of the POU-specific domain of the neuronal factors on their native response elements appears to be opposite that of Pit-1 and Oct-1. Members of POU-III (Brn-2) class exhibit remarkable flexibility in DNA site recognition (tolerating core motifs spaced by 0, 2, or 3 nucleotides), whereas POU-IV (Brn-3) class is highly constrained (tolerating core motifs with a spacing of 3 nucleotides). The molecular determinant of the constraint in DNA site selection appears to be imparted by 3 amino acid residues in the amino-terminal basic region in concert, with helix 2 of the POU homeo domain which together are involved in minor groove and possibly phosphate backbone contacts. Similar mechanisms may underlie differential flexibility in spacing and orientation for diverse families of transcription factors.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
DNA binding  IDA 61700 RGD 
DNA-binding transcription factor activity  TAS 61700 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Pou3f2  (POU class 3 homeobox 2)


Additional Information