RGD Reference Report - The structure, expression, and properties of additional members of the protein kinase C family. - Rat Genome Database

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The structure, expression, and properties of additional members of the protein kinase C family.

Authors: Ono, Y  Fujii, T  Ogita, K  Kikkawa, U  Igarashi, K  Nishizuka, Y 
Citation: Ono Y, etal., J Biol Chem 1988 May 15;263(14):6927-32.
RGD ID: 61695
Pubmed: PMID:2834397   (View Abstract at PubMed)

In rat brain three members of the protein kinase C family encoded by cDNAs termed delta, epsilon, and zeta were newly identified by molecular cloning and sequence analysis. The new members have a common structure that is closely related to but clearly distinct from the four members of the family previously isolated having alpha-, beta I-, beta II-, and gamma-sequences, although the zeta-cDNA available at present does not appear to contain a complete reading frame for protein kinase C. The delta-, epsilon-, and zeta-cDNAs all encode a characteristic cysteine-rich sequence and protein kinase domain sequence, both of which are highly homologous among the protein kinase C family. However, the new members lack one of the conserved regions that is present in alpha-, beta I-, beta II-, and gamma-sequences. An additional cDNA clone termed epsilon' was isolated, which is identical with epsilon-cDNA except for a short sequence at the 5'-terminal end region. The two members having delta- and epsilon-sequences were expressed in COS 7 cells, and partially purified and characterized. The enzymes having delta- and epsilon-sequences depend on phospholipid and diacylglycerol for the enzymatic activity, but their properties slightly differ from the previously known members of protein kinase C. Northern blot analysis suggests that the new members of protein kinase C exist in the brain and some other tissues.

Objects referenced in this article
Gene Prkcd protein kinase C, delta Rattus norvegicus
Gene Prkce protein kinase C, epsilon Rattus norvegicus
Gene Prkcz protein kinase C, zeta Rattus norvegicus

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