RGD Reference Report - A novel neuronal P2x ATP receptor ion channel with widespread distribution in the brain. - Rat Genome Database

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A novel neuronal P2x ATP receptor ion channel with widespread distribution in the brain.

Authors: Seguela, P  Haghighi, A  Soghomonian, JJ  Cooper, E 
Citation: Seguela P, etal., J Neurosci 1996 Jan 15;16(2):448-55.
RGD ID: 61682
Pubmed: PMID:8551329   (View Abstract at PubMed)
PMCID: PMC6578647   (View Article at PubMed Central)

There is strong evidence that ATP acts as an excitatory neurotransmitter in the periphery, yet little is known about fast central ATP-mediated transmission. We report here the molecular cloning of a novel neuronal ionotropic ATP receptor of the P2x subtype (P2x3) isolated from rat brain. This central P2x channel subunit has significant amino acid homology with two recently cloned ATP-gated channels from rat smooth muscle (47%) and pheochromocytoma PC12 cells (37%). P2x3 receptor contains the characteristic 10 conserved cysteines of ATP-gated channels, a putative extracellular region homologous to the Walker type A motif found in various nucleotide-binding proteins, and two potential sites for phosphorylation by protein kinase C. Homomeric receptor P2x3 channels expressed in Xenopus oocytes produce rapid cation-selective purinergic currents that are potentiated by zinc ions and reversibly blocked by the P2x antagonists suramin, Reactive Blue 2, and pyridoxalphosphate-6-axophenyl-2U,4U-disulfonic acid. P2x3-receptor subunit mRNA is found in the Purkinje cells and the granule cells of the cerebellum as well as in CA3 pyramidal cells of the hippocampus that are innervated by zinc-rich axon terminals of mossy fibers. Our results suggest that fast excitatory synaptic transmission mediated by zinc-sensitive ATP-gated channels is widespread in mammalian brain.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
calcium ion transmembrane transport involved_inIDA 61682PMID:8551329UniProt 
cellular response to ATP involved_inIDA 61682PMID:8551329UniProt 
cellular response to zinc ion involved_inIDA 61682PMID:8551329UniProt 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
plasma membrane located_inIMP 61682PMID:8551329UniProt 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
extracellularly ATP-gated monoatomic cation channel activity enablesIDA 61682PMID:8551329UniProt 

Objects Annotated

Genes (Rattus norvegicus)
P2rx4  (purinergic receptor P2X 4)


Additional Information