RGD Reference Report - Isolation of MEK5 and differential expression of alternatively spliced forms. - Rat Genome Database

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Isolation of MEK5 and differential expression of alternatively spliced forms.

Authors: English, JM  Vanderbilt, CA  Xu, S  Marcus, S  Cobb, MH 
Citation: English JM, etal., J Biol Chem 1995 Dec 1;270(48):28897-902.
RGD ID: 61658
Pubmed: PMID:7499418   (View Abstract at PubMed)

The prototype mitogen-activated protein (MAP) kinase module is a three-kinase cascade consisting of the MAP kinase, extracellular signal-regulated protein kinase (ERK) 1 or ERK2, the MAP/ERK kinase (MEK) MEK1 or MEK2, and the MEK kinase, Raf-1 or B-Raf. This and other MAP kinase modules are thought to be critical signal transducers in major cellular events including proliferation, differentiation, and stress responses. To identify novel mammalian MAP kinase modules, polymerase chain reaction was used to isolate a new MEK family member, MEK5, from the rat. MEK5 is more closely related to MEK1 and MEK2 than to the other known mammalian MEKs, MKK3 and MKK4. MEK5 is thought to lie in an uncharacterized MAP kinase pathway, because MEK5 does not phosphorylate the ERK/MAP kinase family members ERK1, ERK2, ERK3, JNK/SAPK, or p38/HOG1, nor will Raf-1, c-Mos, or MEKK1 highly phosphorylate it. Alternative splicing results in a 50-kDa alpha and a 40-kDa beta isoform of MEK5. MEK5 beta is ubiquitously distributed and primarily cytosolic. MEK5 alpha is expressed most highly in liver and brain and is particulate. The 23 amino acids encoded by the 5' exon in the larger alpha isoform are similar to a sequence found in certain proteins believed to associate with the actin cytoskeleton; this alternatively spliced modular domain may lead to the differential subcellular localization of MEK5 alpha.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
MAPK cascade  ICGO:000646861658 RGD 
protein phosphorylation  IDA 61658 RGD 

Molecular Function
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
ATP binding  IDA 61658 RGD 
MAP kinase kinase activity  ICGO:000467261658 RGD 
protein kinase activity  IDA 61658 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Map2k5  (mitogen activated protein kinase kinase 5)


Additional Information