In the present report, we describe the construction of a rat genetic linkage map based on anchor markers mapped in several hundred F2 progeny. The rats were derived from an intercross between the GK rat, a model for type 2 diabetes (Goto et al. 1975), and the F344 rat. Approximately 1200 microsatellite markers were PCR amplified, and 543 of these were informative in the GK/F344 cross and could be amplified readily. Most microsatellites were identified by screening genomic libraries and the Genebank database for repeated sequences (Jacob et al. 1995). Additional markers were also obtained from the Rat Map Database (http://www.ratmap.gu.se/).
[Abstract created from article by RGD Staff]