RGD Reference Report - Proteomic analysis of colonic mucosa in a rat model of irritable bowel syndrome. - Rat Genome Database

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Proteomic analysis of colonic mucosa in a rat model of irritable bowel syndrome.

Authors: Ding, Y  Lu, B  Chen, D  Meng, L  Shen, Y  Chen, S 
Citation: Ding Y, etal., Proteomics. 2010 Jul;10(14):2620-30.
RGD ID: 5686874
Pubmed: PMID:20461717   (View Abstract at PubMed)
DOI: DOI:10.1002/pmic.200900572   (Journal Full-text)

Irritable bowel syndrome (IBS) is one of the most common functional disorders of the gastrointestinal tract. It is characterized by abdominal pain and changes in bowel habits. Various studies have investigated the pathophysiologic processes underlying IBS, but the mechanism remains poorly understood. In the present study, we established an IBS model and identified differentially expressed proteins in colon tissue of IBS rats compared with healthy controls by 2-D gel electrophoresis, MALDI-TOF-MS, and Western blot analysis. Our results showed that 13 of the 1396 protein spots on 2-D gel were differently expressed between the IBS and control groups. Ontological analysis of these proteins revealed primary roles in catalytic activity (protein disulfide-isomerase A3, glyoxalase I, cathepsin S, alpha-enolase), structural support (cytokeratin 8), antioxidant activity (peroxiredoxin-6), protein binding (transgelin, serpin peptidase inhibitor B5), and signal transduction (40S ribosomal protein SA). Protein disulfide-isomerase A3 and cytokeratin 8 overexpression in IBS were confirmed by Western blot. The findings indicate that multiple proteins are involved in IBS processes that influence intestinal tract immunity, inflammation, and nerve regulation. Our study provides useful candidate genes and proteins for further investigation.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
irritable bowel syndrome  ISORpsa (Rattus norvegicus)5686874; 5686874 RGD 
irritable bowel syndrome  IEP 5686874 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Rpsa  (ribosomal protein SA)

Genes (Mus musculus)
Rpsa  (ribosomal protein SA)

Genes (Homo sapiens)
RPSA  (ribosomal protein SA)


Additional Information