RGD Reference Report - Increased heat-shock protein 90 expression contributes to impaired adaptive cytoprotection in the gastric mucosa of portal hypertensive rats. - Rat Genome Database

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Increased heat-shock protein 90 expression contributes to impaired adaptive cytoprotection in the gastric mucosa of portal hypertensive rats.

Authors: Tominaga, M  Ohta, M  Kai, S  Iwaki, K  Shibata, K  Kitano, S 
Citation: Tominaga M, etal., J Gastroenterol Hepatol. 2009 Jun;24(6):1136-41. Epub 2009 Mar 11.
RGD ID: 5686823
Pubmed: PMID:19383083   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1440-1746.2008.05763.x   (Journal Full-text)

BACKGROUND AND AIMS: Portal hypertensive (PHT) gastropathy results in an increased susceptibility to damage. Adaptive cytoprotection against ethanol-induced damage is impaired in the gastric mucosa of rats with portal hypertension. Excessive nitric oxide (NO) production occurs in portal hypertension and is mediated in part via heat-shock protein (Hsp)90 production. The aim of this study was to investigate the relation between adaptive cytoprotection after exposure to ethanol and gastric expression of Hsp90 in PHT rats. METHODS: Portal hypertension was induced in rats by staged portal vein occlusion. Adaptive cytoprotection to 70% ethanol was evaluated by assessing the injury index of the gastric mucosa with or without pretreatment with 10% ethanol. Expression of Hsp90 mRNA was evaluated by real-time polymerase chain reaction, and expression of Hsp90 protein was evaluated by western blotting. The effect of Hsp90 inhibition in PHT rats was evaluated by administration of geldanamycin. RESULTS: Gastric Hsp90 mRNA expression in PHT rats was significantly less than that in sham-operated (SO) controls. However, after 10% ethanol pretreatment, Hsp90 mRNA expression was significantly greater in PHT rats than in SO controls. In PHT rats, gastric Hsp90 protein expression after 10% ethanol pretreatment was significantly greater than that without the pretreatment. However, the pretreatment had no effect on the injury index compared to SO rats. Administration of geldanamycin prior to 10% ethanol pretreatment significantly decreased the injury index in response to 70% ethanol in the PHT rats. CONCLUSIONS: These results show that 10% ethanol pretreatment markedly increases gastric Hsp90 expression in PHT rats. Excessive production of Hsp90 may contribute impaired adaptive cytoprotection.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
portal hypertension  ISOHsp90aa1 (Rattus norvegicus)5686823; 5686823mRNA:decreased expression:stomach (rat)RGD 
portal hypertension  IEP 5686823mRNA:decreased expression:stomach (rat)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Hsp90aa1  (heat shock protein 90 alpha family class A member 1)

Genes (Mus musculus)
Hsp90aa1  (heat shock protein 90, alpha (cytosolic), class A member 1)

Genes (Homo sapiens)
HSP90AA1  (heat shock protein 90 alpha family class A member 1)


Additional Information