RGD Reference Report - Role of Hsp90 in biogenesis of the beta-cell ATP-sensitive potassium channel complex. - Rat Genome Database

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Role of Hsp90 in biogenesis of the beta-cell ATP-sensitive potassium channel complex.

Authors: Yan, FF  Pratt, EB  Chen, PC  Wang, F  Skach, WR  David, LL  Shyng, SL 
Citation: Yan FF, etal., Mol Biol Cell. 2010 Jun 15;21(12):1945-54. Epub 2010 Apr 28.
RGD ID: 5686755
Pubmed: PMID:20427569   (View Abstract at PubMed)
PMCID: PMC2883939   (View Article at PubMed Central)
DOI: DOI:10.1091/mbc.E10-02-0116   (Journal Full-text)

The pancreatic beta-cell ATP-sensitive potassium (K(ATP)) channel is a multimeric protein complex composed of four inwardly rectifying potassium channel (Kir6.2) and four sulfonylurea receptor 1 (SUR1) subunits. K(ATP) channels play a key role in glucose-stimulated insulin secretion by linking glucose metabolism to membrane excitability. Many SUR1 and Kir6.2 mutations reduce channel function by disrupting channel biogenesis and processing, resulting in insulin secretion disease. To better understand the mechanisms governing K(ATP) channel biogenesis, a proteomics approach was used to identify chaperone proteins associated with K(ATP) channels. We report that chaperone proteins heat-shock protein (Hsp)90, heat-shock cognate protein (Hsc)70, and Hsp40 are associated with beta-cell K(ATP) channels. Pharmacologic inhibition of Hsp90 function by geldanamycin reduces, whereas overexpression of Hsp90 increases surface expression of wild-type K(ATP) channels. Coimmunoprecipitation data indicate that channel association with the Hsp90 complex is mediated through SUR1. Accordingly, manipulation of Hsp90 protein expression or function has significant effects on the biogenesis efficiency of SUR1, but not Kir6.2, expressed alone. Interestingly, overexpression of Hsp90 selectively improved surface expression of mutant channels harboring a subset of disease-causing SUR1 processing mutations. Our study demonstrates that Hsp90 regulates biogenesis efficiency of heteromeric K(ATP) channels via SUR1, thereby affecting functional expression of the channel in beta-cell membrane.

Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

Objects Annotated

Genes (Rattus norvegicus)
Hsp90aa1  (heat shock protein 90 alpha family class A member 1)
Hsp90ab1  (heat shock protein 90 alpha family class B member 1)
Kcnj11  (potassium inwardly-rectifying channel, subfamily J, member 11)


Additional Information