RGD Reference Report - The use of multiplexed MRM for the discovery of biomarkers to differentiate iron-deficiency anemia from anemia of inflammation. - Rat Genome Database

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The use of multiplexed MRM for the discovery of biomarkers to differentiate iron-deficiency anemia from anemia of inflammation.

Authors: Domanski, D  Cohen Freue, GV  Sojo, L  Kuzyk, MA  Ratkay, L  Parker, CE  Goldberg, YP  Borchers, CH 
Citation: Domanski D, etal., J Proteomics. 2011 Nov 26.
RGD ID: 5685688
Pubmed: PMID:22146476   (View Abstract at PubMed)
DOI: DOI:10.1016/j.jprot.2011.11.022   (Journal Full-text)

In this study we demonstrate the use of a multiplexed MRM-based assay to distinguish among normal (NL) and iron-metabolism disorder mouse models, particularly, iron-deficiency anemia (IDA), inflammation (INFL), and inflammation and anemia (INFL+IDA). Our initial panel of potential biomarkers was based on the analysis of 14 proteins expressed by candidate genes involved in iron transport and metabolism. Based on this study, we were able to identify a panel of 8 biomarker proteins: apolipoprotein A4 (APO4), transferrin, transferrin receptor 1, ceruloplasmin, haptoglobin, lactoferrin, hemopexin, and matrix metalloproteinase-8 (MMP8) that clearly distinguish among the normal and disease models. Within this set of proteins, transferrin showed the best individual classification accuracy over all samples (72%) and within the NL group (94%). Compared to the best single-protein biomarker, transferrin, the use of the composite 8-protein biomarker panel improved the classification accuracy from 94% to 100% in the NL group, from 50% to 72% in the INFL group, from 66% to 96% in the IDA group, and from 79% to 83% in the INFL+IDA group. Based on these findings, validation of the utility of this potentially important biomarker panel in human samples in an effort to differentiate IDA, inflammation, and combinations thereof, is now warranted. This article is part of a Special Issue entitled: Genome regulations and Genetic diversity.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
anemia  ISOApoa4 (Mus musculus)5685688; 5685688associated with Inflammation and protein:increased expression:blood plasma (mouse)RGD 
anemia  IEP 5685688associated with Inflammation and protein:increased expression:blood plasma (mouse)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Apoa4  (apolipoprotein A4)

Genes (Mus musculus)
Apoa4  (apolipoprotein A-IV)

Genes (Homo sapiens)
APOA4  (apolipoprotein A4)


Additional Information