RGD Reference Report - Screening disease-associated proteins from sera of patients with rheumatoid arthritis: a comparative proteomic study. - Rat Genome Database

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Screening disease-associated proteins from sera of patients with rheumatoid arthritis: a comparative proteomic study.

Authors: Li, TW  Zheng, BR  Huang, ZX  Lin, Q  Zhao, LK  Liao, ZT  Zhao, JJ  Lin, ZM  Gu, JR 
Citation: Li TW, etal., Chin Med J (Engl). 2010 Mar 5;123(5):537-43.
RGD ID: 5685646
Pubmed: PMID:20367977   (View Abstract at PubMed)

BACKGROUND: Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammation at the synovial membrane. Although great progress has been made recently in exploring the etiology and pathogenesis of RA, its molecular pathological mechanism remains to be further defined and it is still a great challenge in determining the diagnosis and in choosing the appropriate therapy in early patients. This study was performed to screen candidate RA-associated serum proteins by comparative proteomics to provide research clues to early diagnosis and treatment of RA. METHODS: Sera isolated from 6 RA patients and 6 healthy volunteers were pooled respectively and high-abundance proteins were depleted by Plasma 7 Multiple Affinity Removal System. The protein expression profiles between the two groups were then compared by two-dimensional gel electrophoresis (2-DE) and the proteins over/under-expressed by more than 3-fold were identified by mass spectrometry analysis. To validate the differential expression levels of the identified proteins between the two groups, ELISA was performed in two of the identified proteins in individual sera from 32 RA patients and 32 volunteers. RESULTS: Eight proteins which over/under-expressed in sera of RA patients were identified. Among them, chain A of transthyretin (TTR) was under-expressed, while serum amyloid A protein, apolipoprotein A (ApoA)-IV, ApoA-IV precursor, haptoglobin 2, ceruloplasmin (Cp), immunoglobulin superfamily 22 and HT016 were over-expressed. ELISA test confirmed that Cp expressed remarkably higher while TTR obviously lower in RA group compared with volunteer group. CONCLUSION: There were 8 identified proteins differentially expressed between RA group and volunteer group, which might be candidate RA-associated proteins and might be promising diagnostic indicators or therapeutic targets for RA.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
intermediate coronary syndrome  IEP 5685646protein:decreased expression:blood plasma (human)RGD 
intermediate coronary syndrome  ISOAPOA4 (Homo sapiens)5685646; 5685646protein:decreased expression:blood plasma (human)RGD 
rheumatoid arthritis  IEP 5685646protein:increased expression:blood serum (human)RGD 
rheumatoid arthritis  ISOAPOA4 (Homo sapiens)5685646; 5685646protein:increased expression:blood serum (human)RGD 

Objects Annotated

Genes (Rattus norvegicus)
Apoa4  (apolipoprotein A4)

Genes (Mus musculus)
Apoa4  (apolipoprotein A-IV)

Genes (Homo sapiens)
APOA4  (apolipoprotein A4)


Additional Information