RGD Reference Report - Loss of imprinting of IGF2 characterises high IGF2 mRNA-expressing type of fibroblast-like synoviocytes in rheumatoid arthritis. - Rat Genome Database

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Loss of imprinting of IGF2 characterises high IGF2 mRNA-expressing type of fibroblast-like synoviocytes in rheumatoid arthritis.

Authors: Martin-Trujillo, A  Van Rietschoten, JG  Timmer, TC  Rodriguez, FM  Huizinga, TW  Tak, PP  Marsal, S  Ibrahim, SM  Dijkmans, BA  Van der Pouw Kraan, TC  Verweij, CL 
Citation: Martin-Trujillo A, etal., Ann Rheum Dis. 2010 Jun;69(6):1239-42. Epub 2009 Jun 24.
RGD ID: 5510001
Pubmed: PMID:19556211   (View Abstract at PubMed)
DOI: DOI:10.1136/ard.2008.106195   (Journal Full-text)

OBJECTIVE: Increased expression of insulin-like growth factor 2 (IGF2) by fibroblast-like synoviocytes (FLS) was associated with low inflammatory synovium of patients with rheumatoid arthritis (RA). The aim of this study was to analyse whether the differential expression of IGF2, whose expression is normally restricted to one allele, is due to activation of the normally suppressed allele. METHODS: IGF2 gene expression of RA FLS was quantified by quantitative real-time PCR. FLS heterozygous for a 3'-untranslated region IGF2 polymorphism were selected to measure the relative contribution of the allelic transcripts by allele-specific transcript quantification assay. Proliferation was determined by [(3)H]thymidine incorporation. RESULTS: IGF2 was shown to contribute to RA FLS proliferation. FLS could be classified in IGF2 high and IGF2 low-expressing cell lines. Allelic IGF2 transcript quantification analysis revealed that in part of the RA FLS the normally suppressed allele was activated, resulting in biallelic expression of the IGF2 gene. Biallelic expression was associated with increased levels of IGF2 mRNA production. CONCLUSION: The findings indicate that the imprinting status of IGF2 might underlie the increased expression of IGF2, which may contribute to autonomous growth of RA FLS of low inflammatory synovial tissues.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
rheumatoid arthritis  IAGP 5510001DNA:loss of imprinting:synovial cellRGD 
rheumatoid arthritis  ISOIGF2 (Homo sapiens)5510001; 5510001DNA:loss of imprinting:synovial cellRGD 

Objects Annotated

Genes (Rattus norvegicus)
Igf2  (insulin-like growth factor 2)

Genes (Mus musculus)
Igf2  (insulin-like growth factor 2)

Genes (Homo sapiens)
IGF2  (insulin like growth factor 2)


Additional Information