RGD Reference Report - Corticotropin releasing factor-distribution in rat intestine and role in neuroprotection. - Rat Genome Database

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Corticotropin releasing factor-distribution in rat intestine and role in neuroprotection.

Authors: Sand, E  Themner-Persson, A  Ekblad, E 
Citation: Sand E, etal., Regul Pept. 2011 Jan 17;166(1-3):68-75. Epub 2010 Aug 27.
RGD ID: 5508197
Pubmed: PMID:20801165   (View Abstract at PubMed)
DOI: DOI:10.1016/j.regpep.2010.08.011   (Journal Full-text)

Aims of the present study were to describe the distribution of corticotropin releasing factor (CRF) immunoreactivity in rat small and large intestines, to quantify the percentage of CRF-immunoreactive (CRF-IR) enteric neurons, to reveal possible CRF immunoreactivity in cultured myenteric neurons from rat ileum and to examine if additions of CRF, urocortin 1 (Ucn1), CRF antagonist or vasoactive intestinal peptide (VIP) affect neuronal survival in vitro. Co-localization of CRF- and VIP-immunoreactivity was examined, as well as a possible interplay between CRF and VIP in neuroprotection. Further we wanted to elucidate if mast cells affect neuronal survival via CRF signaling. Networks of CRF-containing nerve cell bodies and fibers were detected in rat intestine. CRF-IR neurons contained to a high degree also VIP. A low number of cultured myenteric neurons was CRF-IR. CRF, Ucn1 or CRF-antagonist did not promote neuronal survival of cultured myenteric neurons, while VIP significantly enhanced neuronal survival. Simultaneous presence of CRF attenuated the VIP mediated increase in neuronal survival. Co-culturing neurons and mast cells resulted in a marked reduction in neuronal survival, not executed via CRF signaling pathways. Conclusion: CRF is present in enteric neurons and counteracts the neuroprotective effect of VIP in vitro.

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
positive regulation of cellular process  IDA 5508197counteracts vasoactive intestinal peptide-induced myenteric neuron survivalRGD 

Cellular Component
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
neuronal cell body  IDA 5508197 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Crh  (corticotropin releasing hormone)


Additional Information