RGD Reference Report - Decreased monocyte human leukocyte antigen-DR expression after severe burn injury: Correlation with severity and secondary septic shock. - Rat Genome Database

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Decreased monocyte human leukocyte antigen-DR expression after severe burn injury: Correlation with severity and secondary septic shock.

Authors: Venet, F  Tissot, S  Debard, AL  Faudot, C  Crampe, C  Pachot, A  Ayala, A  Monneret, G 
Citation: Venet F, etal., Crit Care Med. 2007 Aug;35(8):1910-7.
RGD ID: 5490160
Pubmed: PMID:17568330   (View Abstract at PubMed)
DOI: DOI:10.1097/01.CCM.0000275271.77350.B6   (Journal Full-text)

OBJECTIVE: Severe thermal injury causes immune dysfunctions involving both pro- and anti-inflammatory mechanisms. It subsequently leads to a state of immune deficiency that shares some similarities with sepsis-induced immunosuppression. A hallmark of the latter is established by decreased monocyte human leukocyte antigen-DR (mHLA-DR) measurements. The main objective of the current study was to characterize the appearance and the duration of low mHLA-DR expression after severe burn as well as to determine its correlation with mortality and septic complications. DESIGN: Observational study. SETTING: Burn unit (intensive care unit) in a university hospital. PATIENTS: Severe burn patients (total burn surface area >30%, n = 14) and healthy individuals (n = 29). INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: Patients were immunologically monitored during 15 days. We quantified mHLA-DR expression with a standardized flow cytometry protocol. Every patient presented with decreased mHLA-DR expression at days 2-3 after burn. Then, from days 4-6, this expression increased in patients who would survive whereas it remained low in nonsurvivors. As early as days 7-10 after burn, patients who were going to develop secondary septic shock exhibited significantly lower mHLA-DR expression in comparison with patients recovering without severe septic complications. Using quantitative reverse transcriptase-polymerase chain reaction, at days 4-6, we found that the RNA level of the nonpolymorphic HLA-DRA chain and the transcription factor class II transactivator were also significantly decreased compared with healthy controls; however, plasma cytokines (interleukin-6, tumor necrosis factor-alpha, and interleukin-10) did not provide any significant prognostic information. CONCLUSIONS: Severe burn injury induced a marked reduction in mHLA-DR expression at both protein and messenger RNA levels. Its persistent decrease was associated with mortality and the development of septic complications.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
toxic shock syndrome  ISOHLA-DRA (Homo sapiens)5490160; 5490160associated with BurnsRGD 
toxic shock syndrome  IEP 5490160associated with BurnsRGD 

Objects Annotated

Genes (Rattus norvegicus)
RT1-Da  (RT1 class II, locus Da)

Genes (Mus musculus)
H2-Ea  (histocompatibility 2, class II antigen E alpha)

Genes (Homo sapiens)
HLA-DRA  (major histocompatibility complex, class II, DR alpha)


Additional Information